荷叶血中移行成分归属的实验研究

Studies on Adscription of Plasma Effective Constituents of Wistar Rat after Administrated with the Extract of Lotus Leaf

目的研究大鼠灌胃荷叶提取物后血中移行成分及其归属。方法通过建立灌胃荷叶提取物后大鼠血浆HPLC指纹图谱,比较给药及未给药大鼠血浆的HPLC指纹图谱,同时根据保留时间判断各谱峰的相关性,进而确认灌胃荷叶提取物后大鼠血中移行成分及其归属。结果给药后血浆中出现了移行成分,其中有原型成分,也有代谢产物。结论对血中移行成分及代谢产物的研究有助于阐明荷叶体内的药效物质基础。

OBJECTIVE To investigate the plasma effective constituents of rat after oral administration with the extract of Lotus leaf.METHODS Based on the established HPLC fingerprints,comparison was carried out among the blank plasma,plasma containing drug,Lotus leaf.Simultaneously,the retention time was an index to evaluate the relation among the fingerprint peaks so as to confirm the original of peaks.RESULTS Components detected after o-ral administration including the original components and its metabolites.CONCLUSION Studying on the constitu-ents and metabolites in plasma is useful to research the effective substances of Chinese medicine of Lotus leaf in vi-vo.