鬼针草植物HPLC指纹图谱研究

Studies on the HPLC Fingerprint of Bidens bipinnata L

目的建立鬼针草植物的HPLC指纹图谱,为鬼针草药材的品种鉴定质量控制提供依据。方法采用迪马Diamonsil 5U(C18,250×4.6mm)色谱柱,以乙腈-0.2%磷酸水溶液为流动相进行梯度洗脱,流速为1.0m L·min-1,检测波长为360nm,柱温为25℃:进样量为20μL。结果建立了10批鬼针草的HPLC指纹图谱,确立了17个共有峰,多数峰可以达到较好分离。并对种间相似度进行了比较。结论该方法稳定性、重复性好,建立的指纹图谱可为鬼针草药材的质量评价提供依据。

OBJECTIVE To establish HPLC fingerprint of Bidens bipinnata L,which was expected to be stand-ards of quality control and identification of the Chinese crude drug.METHODS The HPLC method was used,chro-matography conditions were Diamonsil 5U （ C18 ,250 ×4.6mm） column with gradient mobile phase of acetonitrile Phosphoric acid.UV detection wavelength was 360nm and the column temperature was 25℃ with the flow rate of 1.0mL·min, the sample injection was 20μL.RESULTS HPLC fingerprints of 10samples of Bidens bipinnata Lwere established.17 mutual peaks were selected as the fingerprint peaks.Among the obtained fingerprints,most of the detected peaks were separated effectively.Samples from different species were compared.CONCLUSION The established HPLC fingerprint has desirable accuracy, repeatability and stability, which can be used for one of the quality control of Bidens bipinnata L.