银杏叶提取物对家兔高胆固醇血症模型脑组织形态学的影响

Effect of the extract of Ginkgo Biloba in brain tissue morphology in rabbit hypercholesteremia model

[目的]通过高胆固醇血症家兔模型观察银杏叶提取物(EGB761)对中枢神经纤维的影响。[方法]以成年雄性新西兰大白兔复制高胆固醇血症模型,实验分为4组:对照组、模型组、阳性药辛伐他汀组、EGB761组。用苏木精-伊红(HE)染色和嗜银染色法观察神经元及神经纤维的形态学改变。[结果]外周胆固醇升高时,模型组海马出现较多神经元变性、坏死,神经纤维缠结等病理改变。EGB761组变性、坏死的神经元明显少于模型组,细胞核形态基本规则,嗜神经现象少见,神经纤维缠结少于模型组。[结论]EGB761可有效保护高胆固醇血症时神经纤维结构的完整性。

Objective] To study the effect of the extract of Ginkgo Biloba 761 （EGB 761） on brain tissue morphology in rabbit hyperc-holesteremia model. [Methods] We built the hypercholesteremia model with male New Zealand white rabbits, which were divided into four groups：the control group, the model group, the simvastatin group, the EGB761 group. Detecting the morphology change of nerve fiber with HE staining and silver staining. [Results] The hippocampus in model group showed neuronal degeneration, necrosis and microfibers tangles while the EGB761 group showed less neuronal degeneration, necrosis and microfibers tangles, and regular nuclear morphometry. [Conclusion] EGB761 can effectively protect the integrity of the structure of nerve fibers in hypercholesterolemia.