阿魏菇多糖的结构分析

Structural Analysis of Polysaccharides from Pleurotus ferulae Lenzi

本文以阿魏菇中分离纯化得到的多糖-阿魏菇多糖（Pleurotus ferulae Lenzi polysaccharide,PFLP）作为研究对象,通过高效凝胶过滤色谱来测定其分子量以及分布,通过气相色谱法（GC）、部分酸水解、高碘酸氧化法、Smith降解法、甲基化反应、红外光谱（IR）、核磁共振氢谱（1HNMR）、刚果红实验和碘-碘化钾实验来分析PFLP的结构。经分离纯化后得到两种纯PFLP组分（PFLP1、PFLP2）,结果分析表明,PFLP1的分子量范围为9400-9900 u;含有鼠李糖、甘露糖、葡萄糖和半乳糖四种单糖,摩尔比为1：1.54：18.6：3.64;PFLP2的分子量范围为9700-10400 u;含有鼠李糖、木糖、甘露糖、葡萄糖和半乳糖五种单糖,摩尔比为1：1.08：0.65：6.76：4.28。PFLP1和PFLP2除了都具有1→2、1→3、1→6糖苷键,还具有1→4糖苷键;都具有典型的多糖红外吸收,是以β-吡喃糖苷为主并且含有蛋白质的糖缀合物的多糖;并且都具有三股螺旋结构,有较长的侧链和分支。

The Pleurotus ferulae Lenzi polysaccharide（PFLP） was isolated and purified to study its components and structure. The relative molecular mass and distribution of PFLP were analyzed byhigh-performance gel filtration chromatography. The structure was analyzed by gas chromatography（GC）, partial acidhydrolysis, periodate oxidation, Smith degradation, methylation, infrared spectroscopy（IR）, 1HNMR, Congo red experiment, and iodine-potassium experiment. Two components, PFLP1 and PFLP2, were obtained through extraction and purification. The result indicated that the relative molecular mass and distribution of PFLP1 was 9400-9900 u. PFLP1 was composed of rhamnose, mannose, glucose, and galactose with a molar ration of 1：1.54：18.6：3.64. The relative molecular mass and distribution of PFLP2 was 9700–10400 u. PFLP2 was composed of rhamnose, xylose, mannose, glucose, and galactose with a molar ration of 1：1.08：0.65：6.76：4.28. Both PFLP1 and PFLP2 contained glycosidic bond of（1→2）,（1→3）,（1→6）, and（1→4）. PFLP1 and PFLP2 possessed typical infrared absorptions of polysaccharides, and the sugar chains were mainly pyranoside with β-configuration and contained a type of protein glycoconjugate. PFLP1 and PFLP2 possessed triplehelical structures containing long side chains and branches.