摘要
在水温21~23℃,pH 8.2~8.5,溶解氧6.00~7.50mg/L的条件下,采用半静水法研究了非离子氨氮和亚硝酸盐氮对全长(1.6±0.2)cm、体质量为(0.11±0.05)g的暗纹东方鲀稚鱼的急性毒性效应。试验结果表明,暗纹东方鲀稚鱼受到非离子氨氮和亚硝酸盐氮胁迫后,先后出现鱼体体色变白、扭曲、侧游、失去平衡、昏迷等中毒症状。随着非离子氨氮和亚硝酸盐氮质量浓度的提高和胁迫时间的延长,暗纹东方鲀稚鱼死亡率逐渐升高,存在明显的剂量效应和时间效应关系。非离子氨氮和亚硝酸盐氮对暗纹东方鲀稚鱼96h半致死质量浓度分别为0.46mg/L(95%置信限0.34~0.64mg/L)和290.12mg/L(95%置信限255.16~329.87mg/L),安全质量浓度分别为0.046 mg/L和29.01mg/L。非离子氨氮和亚硝酸盐氮对暗纹东方鲀稚鱼具有一定毒性,且非离子氨氮毒性大于亚硝酸盐氮毒性。
The acute toxicity of non-ionized ammonia and nitrite to postlarvae of puffer Takifugu fasciatus with total length of(1.6 ± 0.2)cm and wet body weight of(0.11 ± 0.05)g was studied by 96 hsemistatic bioassays under conditions of water temperature 21—23℃,pH 8.0—8.5and dissolved oxygen level of 6.00—7.50 mg/L.The postlarvae exposed to the non-ionized ammonia and nitrite showed a series of poisoning symptoms,such as body whitening and bending,and loss of equilibrium and had elevated mortality gradually with increase in the toxicant concentration and exposure elapse,with dose-time dependent.The 96 h median lethal concentration(96hLC50)values of the postlarvae was found to be 0.46 mg/L(95% confidence limits:0.34—0.64mg/L)with safe concentration(SC)values of 0.046mg/L for non-ionized ammonia and 290.12mg/L(95%confidence limits:255.16—329.87mg/L)with SC of 29.01mg/L for nitrite.The findings demonstrated that both non-ionized ammonia and nitrite were toxic to the puffer and that non-ionized ammonia had stronger toxicity than nitrite.
出处
《水产科学》
CAS
CSCD
北大核心
2015年第3期135-139,共5页
Fisheries Science
基金
国家重大星火计划项目(2012GA690001)
江苏省科技支撑计划(农业)项目(DE2013354)