择时有氧运动对2型糖尿病大鼠凝血纤溶系统的影响

The Effects of Aerobic Chronoexercising on Cruor-fibrinolysis System of T2DM Rats

目的:基于时间生物学理论,依据正常血糖昼夜节律,探讨择时有氧运动对12DM大鼠凝血纤溶系统特异性指标P-selectin、TAT、t-PA、PAI-1水平的影响。方法:将80只4周龄雄性SD大鼠随机分为健康空白组(N=12,H组)和糖尿病造模组(N=68,G组)。4周后将G组造模成功的56只T2DM大鼠随机分为糖尿病对照组(C组),6:00运动组(SM组),12:00运动组(SN组)和18:00运动组(SE组)。第6周末,各组禁食12h后尾静脉取血测空腹血糖,心尖采血分离血浆待测。结果:1.6周择时有氧运动干预后,SN、sE组FPG水平低于SM组(P<0.05);FINS水平运动三组较C组均有降低(P<0.01),但运动组组间比较无明显差异(P>0.05);SE组Homa-IR水平显著低于SM组(P<0.05),运动三组IAI水平无明显变化。2.血浆P-selecfin、TAT、PAI-1水平运动组较C组降低(P<0.01),而血浆t-PA水平升高(P<0.01)。运动组组间比较,SE组升高最明显,且差异具有极显著性(P<0.01)。结论:1.T2DM大鼠血糖升高,血糖波动节律紊乱,胰岛素抵抗,凝血纤溶系统功能失衡,血液呈高凝状态。2.六周择时有氧运动可有效降低血糖,缓解胰岛素抵抗。18:00或12:00效果优于6:00,但对血糖水平波动的生物周期节律无调节作用。3.六周择时有氧运动可以改善T2DM大鼠的血液高凝状态,改善血小板功能,促使凝血纤溶系统功能趋于平衡,保证血流畅通。18:00有氧运动干预效果好于6:00。

Objective： Based on the theory of chronobiology and patterns of normal blood glucose fluctuations, this paper explores the impact of aerobic chronoexercising on specificity index of T2DM rats cruor - fibrino]ysis system such as P- selectin, TAT, t -PA and PAl -1. Methods：Randomly divides 80 healthy, 4 -week -old, male SD rats into the health control group （N = 12, H） and diabetic model group （N = 68, G）. 4 weeks later, randomly divide the successfully modeled 56 T2DM rats into diabetic control group （N = 14, C） ,6：00 group （N = 14, the SM group）, 12：00 group （N = 14, SN） and 18：00 （N = 14, SE）. On the 6th weekend, each group is fasted for 12 hours then the FPG of the tail vein is measured. Separated plasma of apex blood is to be measured. Results：l. After six weeks of aerobic chronoexercising, FPG level of SN, SE group is lower than that of SM group （ P 〈 0.05） ; Compared to C group, FINS of S groups all decrease （ P 〈 0.01 ）, but no significant difference within the SM, SN, and SE groups （ P 〉 0.05 ） ; Homa - IR of SE group is significantly lower than that of SM group （ P 〈 0.05 ）, but IAI have no obvious changes within the S groups. 2. Compared to C group, plasma P - selectin, TAT and PAl - 1 levels in S groups were decrease （ P 〈 0.01 ）, while plasma t - PA increased （ P 〈 0.01 ）. Within the S groups, SE group demonstrate the most significant increase （ P 〈 0. 01 ） and the differences within is very obvious. Conclusions： 1. FPG had increased in T2DM rats but circadian rhythm was disorder, accompanied with insulin resistance; function imbalance of cruor -fibrinolysis system and blood in hypercoagulable state. 2. The six weeks of aerobic chronoexercising can effectively reduce the level of FPG and alleviate insulin resistance and the effect of aerobic chronoexercising in 18：00 or 12：00 is better than that in 6：00, but it has no effect on biological rhythm of the fluctuations in blood glucose. 3. The six weeks of aerobic chronoexercising can improve blood hypercoagulability of T2DM rat, function of platelet and function balance of cruor -fibrinolysis system, ensuring smooth flow of blood. The effect of aerobic chronoexereising in 18：00 is better than that in 6：00.