HPLC法确定马鞭草中毛蕊花苷的提取条件

Determination for Extraction Condition of Verbascoside in Verbena oficinals L. by HPLC

为了确定马鞭草中毛蕊花苷的提取条件,采用HPLC监测,比较提取温度、提取溶剂、提取次数等因素。结果表明,最佳提取条件为:以80%乙醇与干燥马鞭草以75∶10(体积(m L)∶重量(g))的比例在65℃提取12个小时,连续提取3次。

To determine the extraction condition of verbascoside in verbena oficinals L.. Three factors,such as extraction temperature,extraction solvent ratio,extracting times,were investigated by HPLC,respectively. The results proved that,the optimum extraction condition showed that a 10- g dried grass was extracted with 75 ml 80% ethanol in a water bath（ temperature was 65 ℃） for 12 hours for three times.