斑马鱼尾加压素Ⅱ受体基因组织表达的研究

The studies of urotensin Ⅱ receptor gene expression in zebrafish tissues

尾加压素Ⅱ受体(UrotensinⅡreceptor,UT)是一种7次跨膜G蛋白偶联受体,是目前发现的具有最强收缩血管作用的尾加压素Ⅱ的特异性受体。斑马鱼中存在4种不同的UT-like基因(uts2r1,uts2r2,uts2r3和uts2r4),分别位于斑马鱼4条不同染色体上。这4种uts2r基因核苷酸序列各不相同,uts2r1位于3号染色体上,其与12号染色体上的uts2r2基因蛋白序列相似度最高为47%,与6号染色体上的uts2r3和16号染色体上的uts2r4蛋白相似度分别是41.6%和33%。通过设计特异引物检测每种uts2r基因在斑马鱼成鱼各组织中的表达情况,结果显示3号染色体上的uts2r1基因在肾脏中表达量最高,6号染色体上的uts2r2基因在脊髓中表达量最高,12号染色体上的uts2r3基因在心脏中表达量最高,而16号染色体上的uts2r4基因在背肌中表达量最高。

Urotensin Ⅱ receptor is a seven-transmembrane G protein-coupled receptor, and it is a specific receptor for urotensin Ⅱ which is the most potent mammalian vasoconstrictor to date. There are four different UT-like genes（uts2r1, uts2r2, uts2r3 and uts2r4） in zebrafish , located on four different chromosomes, while the nucleotide sequence of these uts2r gene varies, UT of chromosome 3 and chromosome 12 shared the highest homology of 47% , and shared 41.6% and 33% homology with the uts2r gene from 6th chromosome and 16th chromosome, respectively. Specific primers were designed for each gene analyzing mRNA expression of different adult fish tissues. Quantitative PCR results showed that uts2rl gene on chromosome 3 has the highest expression level in the kidney, uts2r2 gene on chromosome 6 has the highest expression level in the spinal cord, and uts2r3 gene on chromosome 12 expressed the highest level in the heart, while the highest peak of uts2r4 mRNA on 16th chromosome expression emerged in the dorsal muscles.