狭叶薰衣草特征指纹图谱研究

Study on HPLC Characteristic Fingerprints of Lavandula Angustifolia

目的建立20批狭叶薰衣草的指纹图谱。方法采用Phenomenex ODS-A色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈和0.036 mol/L磷酸二元梯度洗脱,流速1.0 m L/min,柱温30℃,检测波长350 nm。通过相似度评价和主成分聚类分析对20批狭叶薰衣草进行归类。结果提取了10个色谱峰为狭叶薰衣草的指纹图谱共有峰,指认了2个色谱峰。20批狭叶薰衣草的相似度在0.9以上,通过主成分聚类分析将20批狭叶薰衣草分为3类。结论该方法操作简便,重复性好,建立的狭叶薰衣草指纹图谱可用于规范和控制狭叶薰衣草药材的质量。

Objective To establish the fingerprints of 20 batches of Lavandula Angustifolia by HPLC. Methods The determination was performed on a Phenomenex ODS-A column （250 mm× 4.6 mm, 5 μm）. The mobile phase was in gradient elute mode with a mixture consisting of acetonitrile and 0.036 mol/L phosphate acid solution. The flow rate was 1.0 mL/min. The temperature was 30 °C. The determine wavelength was 350 nm. The fingerprints of 20 batches of Lavandula Angustifolia were compared and classified by similarity evaluation, cluster analysis, and principal composition analysis. Results Totally 10 chromatographic peaks were extracted as the common peaks of Lavandula Angustifolia, and 2 peaks were identified. The similarity degrees of the 20 batches of Lavandula Angustifolia were above 0.9. All the batches of Lavandula Angustifolia were classified into 3 categories. Conclusion The method is simple and reproducible, and can be used for the standardization and quality control of Lavandula Angustifolia.