辛伐他汀干预急性心肌梗死后gp130表达与左室重塑的关系

Effect of simvastatin on gp130 expression and ventricular remolding after acute myocardial infarction in rats

目的:研究辛伐他汀干预急性心肌梗死(AMI)后左室非梗死区(LVNIZ)糖蛋白链细胞因子受体亚单位(gp)130(gp130)表达及其与左室重塑(LVRM)的关系。方法:40只SD雄性大鼠平分为4组:心肌梗死组、心肌梗死+辛伐他汀组(辛伐他汀40 mg·kg-1·d-1灌胃)、假手术组和正常组。4周后用RT-PCR测定LVNIZ gp130mRNA基因表达和用Western Blot法测定LVNIZ蛋白合成水平,同时测定左室非梗死区肌细胞横截面积,和心肌胶原容积分数。结果:4周后心肌梗死组LVNIZ gp130mRNA表达和蛋白合成水平于明显高于正常组和假手术组(P<0.05)。心肌梗死+辛伐他汀组LVNIZ gp130mRNA表达和蛋白合成水平明显低于心肌梗死组(P<0.05)。同时与正常组及假手术组比较心肌梗死组心肌细胞横截面积(CA),I,III胶原容积分数(CVFI,CVFIII)均明显增加(P<0.05)。随着辛伐他汀的干预与心肌梗死组比较,心肌梗死+辛伐他汀组心肌细胞横截面积(CA),I,III胶原容积分数(CVFI,CVFIII),均明显改善(P<0.05)。结论:大鼠AMI后LVNIZ gp130mRNA和蛋白的过度表达与AMI后LVRM的发生有联系,辛伐他汀可改善LVRM,其机制可能与抑制gp130基因过度表达与蛋白合成有关。

Objective ：To determine the effect of simvastatin on gpl30 expression in left ventricular non infarct zone （LVNIZ） and related left ventricular remodeling （LVRM） after acute myocardial infarction （AMI）. Methods ： Male SD rats （n = 40） were divided into four groups ： AMI, AMI ＋ simvastatin, sham-operated, and normal groups. Four weeks after AMI, gp130 mRNA and protein expression in LVNIZ were determined by RT-PCR and Western blot. Moreover, the cross-sectional area of cardiocytes and the collagen volume fraction （CVF） were determined. Results： Compared with normal and sham-operated rats, the expression of gp130 mRNA and protein in LVNIZ significantly increased 4 weeks after AMI （P 〈 0. 05）. Compared with AMI control, simvastatin decreased gp130 mRNA and protein expression. Moreover, cardiocyte cross-sectional area （CA） and CVF in LVNIZ were significantly increased （ P 〈 0. 05 ） after AMI ; the increased CA and CVF were alleviated by simvastatin （P 〈 0.05 ）. Conclusion： Gp130 mRNA and protein are overexpressed in LVNIZ after AMI. Simvastatin prevents LVRM which may be partly through depressing gp130 mRNA and protein expression.