MTA1基因表达与下咽癌颈淋巴结转移的关系

The Correlation of the Expression of the MTA1 Gene with Cervical Lymph Node Metastasis in Hypopharyngeal Squamous Cell Carcinoma

目的:探讨下咽癌组织中转移候选基因(metastasis-associated gene1,MTA1)的表达与颈淋巴结转移的关系,阐明该基因可能是下咽癌淋巴结转移的分子机制之一。方法:应用逆转录-聚合酶链式反应(reverse transcription polymerase chain reaction,RT-PCR)检测17例下咽癌(11例伴颈淋巴结转移、6例不伴颈淋巴结转移),8例癌旁正常黏膜中MTA1mRNA的表达。结果:RT-PCR结果显示在11例下咽癌转移组的癌组织中,MTA1 mRNA表达阳性率为90.9%(10/11);在6例不伴颈淋巴结转移的其它下咽癌组织及8例癌旁正常黏膜中MTA1 mRNA的表达率分别为33.3%(2/6)和0%。转移组的表达率与其他2组之间差异有统计学意义(P<0.01)。结论:MTA1 mRNA的表达与下咽癌颈淋巴结转移密切相关,提示MTA1 mRNA的检测可作为下咽癌颈淋巴结转移的诊断指标。

Objective： To examine the mRNA expression levels of the metastasis-associated gene 1（ MTA1） in hypopharyngeal squamous cell carcinoma（ HSCC）,and thus to evaluate the relevance of the expression of this gene to the progression of HSCC to the metastatic state. Methods： A total of 17 surgically resected primary HSCC（ 11 HSCC with cervical lymph node metastasis,6 HSCC without cervical lymph node metastasis） and 8 normal adjacent mucosa was examined for mRNA expression of MTA1 by reverse transcription-polymerase chain reaction（ RT-PCR）. Results： The positive expression rate of MTA1 mRNA in 11 HSCC with cervical lymph node metastasis was 90. 9%,but the positive expression rate of MTA1 mRNA in 6 HSCC without cervical lymph node metastasis was 33. 3% and no expression of MTA1 mRNA was observed in normal adjacent mucosa. The positive expression rate of HSCC with cenvical lymph node metastasis was higher than that in the other 2 groups（ P〈0. 01）. Conclusions： MTA1 gene is strongly related to cervical lymph node metastasis of HSCC,and may serve as a meaningful advance-diagnosis factor. Expression of the MTA1 gene is frequent events related to cervical lymph node metastasis of HSCC.