高效液相色谱法检测中药有效成分研究

HPLC Determination of Effective Components of Chinese Herbal Medicine

本研究采用高效液相色谱法(HPLC),对苦杏仁(产地甘肃)、熟地黄(产地河南)、葛根(产地陕西)和何首乌(产地陕西)四种中药的有效成分进行检测,为其质量评价提供依据。结果表明,以十八烷基硅烷键合硅胶为填充剂的色谱柱,4种中药有效成分呈现极强的相关性,其相关系数为0.9 999。苦杏仁苷(C20H27O11)、毛蕊花糖苷(C29H-36O15)、葛根素(C21H20O9)和2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷(C20H22O9)的平均回收率分别达到了97.39%、97.9%、96.7%和97.25%。苦杏仁苷、熟地黄、葛根和何首乌的有效成分含量分别为2.85%、0.059%、3.228%和2.02%。说明HPLC法检测中药有效成分操作快速,结果准确,精密度高,稳定性好,所测定的4种中药材有效成分的含量均符合2010年版药典规定。

This study aimed to determinate the content of four kinds of Chinese herbal medicine by HPLC. Gradient elution was applied in HPLC analysis. Octadecylsilane chemically bonded silica is the chromatographic column,velocity 1 mL · min-1 , column temperature was 25 ℃, effective ingredient a method for determination of different active ingredients in four kinds of Chinese herbal medicine by HPLC simultaneously and provide the basis for quality testing. The sample size showed respectively a good linear relationship （r=0. 999 9）. The average recovery of C20 H27 O11 is 97.39 %, the C29 H36 O15 is 97.9%, the C21 H20 09 is 96.7% and the C20H2209 is 97.25%. Therefore, the method is simple, accurate and with good reproducibility.