葡萄酒中26种添加剂的高效液相色谱-高分辨质谱筛查法

Screening of 26 Kinds of Additives in Wines by High Performance Liquid Chromatography-High Resolution Mass Spectrometry

建立了一种高效液相色谱-四极杆/静电场轨道阱高分辨质谱同时筛查葡萄酒中26种添加剂（包括防腐剂、抗氧化剂、甜味剂和合成着色剂）的方法。葡萄酒经甲醇-水溶液（1∶9,V/V）稀释后,采用超高效C18柱分离,高分辨质谱全扫描模式提取目标化合物的精确质量数,外标法定量。在0.1-5mg/L范围内,26种添加剂的线性关系良好,线性相关系数均大于0.99。干红葡萄酒和干白葡萄酒基质中,2、4、20mg/kg 3个添加水平的回收率均在78.5%-118.8%之间,相对标准偏差小于12.7%,定量限为2mg/kg。该方法的前处理快速、简单,高分辨质谱定量结果的准确度和精密度高,可用于葡萄酒中添加剂的筛查。

The method for screening of 26 kinds of additives in wine was developed by liquid chromatography-quadrupole/orbitrap high resolution mass spectrometry. The addilives were separated by C18 high performance column after the wines were diluted by methanol-water mixtures （1 ： 9, V/V）. This method was quantitatived by external standard method using full scan mode of high resolution mass spectrometer extracting accurate mass extraction of the target compound. Good linearity is obtained in the range of 0. 1-5 mg/L for 26 kinds of additives with the linear correlation coefficients greater than 0. 99. Satisfactory recoveries range from 78.5% to 118.8% at three levels of 2, 4 and 20 mg/kg are gained in dry red and white wines with the relative standard deviation less than 12.7%. The limit of quamitation （LOQ） is 2 mg/kg. This method is suitable for screening of 26 kinds of additives in wines by its fast and simple preproeessing with high accuracy and precision of high resolution mass spectrometry.