芍药苷对血虚肝郁证模型大鼠海马cGMP含量及其合成与水解酶的影响

Effects of paeoniflorin on the content, synthesis and hydrolase of cGMP in the hippocampus of rats with blood deficiency and liver depression syndrome

目的:研究血虚肝郁证大鼠海马c GMP含量及其合成酶s GCα2、s GCβ1和水解酶PDE2A、PDE5A、PDE9A m RNA表达与芍药苷的干预作用。方法:分为空白组、模型组、40mg/kg芍药苷组、20mg/kg芍药苷组,造模第1天开始给药,空白组、模型组给等量蒸馏水,其余各组给予相应药物,共造模21d。治疗结束后,取脑组织,剥离海马,采用格里斯法(Griess法)测定NO含量、放射免疫法(RIA)测定c GMP含量以及实时荧光定量聚合酶链反应法(RT-q PCR)检测s GCα2、s GCβ1、PDE2A、PDE5A、PDE9A m RNA的表达。结果:血虚肝郁证模型大鼠海马的NO、c GMP含量升高且s GCα2、s GCβ1、PDE2A、PDE5A m RNA的表达增加(P<0.05,P<0.01),40mg/kg芍药苷能够降低NO、c GMP含量(P<0.01,P<0.001)且下调其合成与分解酶的表达。结论:芍药苷能够降低血虚肝郁证模型大鼠海马NO、c GMP的含量,且下调s GCα2、s GCβ1、PDE2A、PDE5A m RNA的表达,其机制与芍药苷影响合成酶与水解酶的变化有关,从两个方面综合作用保持其含量的动态平衡。

Objective： To observe the influence of Paeortiftorin on the content of cGMP and the mRNA expressions of cGMP synthases sGC a 2, sGC 13 1 and hydrolases PDE2A, PDE5A, PDE9A in the hippoeampus of rats with blood deficiency and liver depression syndrome. Methods： SD rats were divided into blank group, model group, 40mg/kg of paeoniflorin group and 20mg/kg of Paeoniflorin group. From the first day of modeling, both treatment groups were received corresponding medicine, while the normal group and the model group were received the same amount of distilled water, lasting for 21 days. On the 21th day, the rat brain tissue and hippocampus tissue were removed. The Griess method was used to detect the content of NO. The content of cGMP was detected by RIA. And, the mRNA expressions of sCJC a 2, sGC 13 1, PDE2A, PDE5A, PDEgA were detected by Real-time PCR. Results： The contents of NO and cGMP were increased in the hippocampus of rats with blood deficiency and liver depression syndrome （P〈0.05, P〈0.01）, as well as the mRNA expressions of sCJC oL 2, sGC [3 1, PDE2A, PDE5A （P〈0.05, P〈0.01）, which were all decreased after treated by 40mg/kg of paeonifiorin （P〈0.01, P〈0.001）. Conclusion： Paeoniflorin can reduce the contents of NO and cGMP and down-regulate the mRNA expressions of sCJC a 2, sGC 13 1, PDE2A, PDE5A, the mechanism of which may be related to influencing the changes of cGMP synthases and hydrolases that comprehensively maintain a dynamic balance in cGMP from two aspects.