清肠愈疡汤对溃疡性结肠炎大鼠血清IL-1β及IL-6的影响

Effects of Qingchang Yuyang Decoction on serum IL-1β and IL-6 in rats with ulcerative colitis

目的:通过观察中药清肠愈疡汤对溃疡性结肠炎模型大鼠一般生理状态、疾病活动指数(DAI)评分、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)产生的影响,探讨其作用机制。方法:将大鼠按体质量随机分为5组,分别为正常组、模型组、清肠组、香连组、5-ASA组,分组饲养12d后,进行DAI评分,ELISA法检测大鼠血清中IL-1β、IL-6的含量。结果:清肠组治疗后DAI评分第4天、第8天、第12天分别为1.75±0.59,0.83±0.31,0.59±0.30,为逐渐降低趋势;大鼠血清中IL-1β与IL-6水平显著下降,分别达到(12.51±0.63)ng/L,(53.72±4.46)ng/L,清肠组与5-ASA组、香连组、模型组比较,均有显著性差异(P<0.05)。结论:清肠愈疡汤可以显著改善清肠组大鼠症状,能够下调大鼠血清中IL-1β、IL-6的水平,减轻大鼠结肠组织的炎症反应,而起到治疗溃疡性结肠炎的作用。

Objective： Observation of the effects of QingchangYuyang Decoction on the general physiological state, disease activity index （DAI） score, IL-1β and IL-6 in serum in ulcerative colitis rat model to explore its mechanism. Methods： According to the bodyweight, the rats were randomly divided into 5 groups, including normal group, model group, Qingchang group, Xianglian group and 5-ASA group. DAI score and the concentrations of IL-I [3 and IL-6 in serum were determined after the rats were fed for 12 days. Results： After the treatment, DAI score was gradually decreased in Qingchang group, with the DAI score as 1.75±0.59, 0.83±0.31 and 0.59±0.30 at the 4th, 8th, 12th day respectively. The concentrations of IL-1β and IL-6 in rats serum were obviously reduced, with the concentrations of IL-1β and IL-6 up to （12.51±0.63）ng/L and （53.72±4.46）ng/L respectively. Compared with Qingchang group, the concentrations of IL-1β and IL-6 in 5-ASA group, Xianglian group and model group, were significant differences （P〈0.05）. Conclusion： Qinchang Yuyang Decoction could significantly improve the symptoms of ulcerative colitis in rats model, that may be related to down-regulation of the level of IL- 1β and IL-6 in serum and relieving the inflammation response in intestinal tissue, playing an important role of treatment for ulcerative colitis.