加味补中益气汤对变应性鼻炎大鼠鼻腔黏膜P物质及P物质受体表达活性的影响

Effects of Jiawei Buzhong Yiqi Decoction on the expression activity of SP and SPR in nasal mucosa of rats with allergic rhinitis

目的:观察加味补中益气汤对变应性鼻炎(AR)模型大鼠鼻腔黏膜P物质(SP)和P物质受体(SPR)表达活性的干预效应。方法:常规方法制作SD大鼠(AR)模型,随机分为模型组、补中益气组、玉屏风组,并另设正常组。药物干预完毕后,处死动物,取鼻腔黏膜组织,甲苯胺蓝染色法观察肥大细胞(MC)密度及形态特征,免疫组化法检测鼻腔黏膜中SP和SPR活性表达水平,比较各组指标差异,分析观察药物的药效学特点。结果:补中益气组的症状积分、MC浸润密度均明显低于模型组和玉屏风组,鼻腔黏膜组织SP和SPR表达活性明显低于模型组(P=0.000)。结论:加味补中益气汤对AR症状的缓解效应,可能是通过抑制鼻腔黏膜SP的释放以及SPR的表达,降低MC浸润程度,减轻组织炎性反应而得以实现。

Objective： To investigate the effects of Jiawei Buzhong Yiqi Decoction on the expression activity of SP and SPR in nasal mucosa of rats with allergic rhinitis （AR）. Methods： Rat model of AR was established by conventional method, and then the rats were randomly divided into 4 groups： model group （MG）, Buzhong Yiqi group （BYG） and Yupingfeng group （YPG） and normal control group （NCG）. All the rats were sacrificed after drug intervention. The density and morphology of mast cells were observed by using toluidine blue staining. Immunohistochemistry was used to test the expression of SP and SPR. Differences among the groups were compared, and pharmacodynamic characteristics of drugs were analyzed. Results： The symptom score and infiltrating density of mast cells of rats in BYG were significantly lower than those in MG and YPG, and the expression activity of SP and SPR was significantly lower than that in MG （P=0.000）. Conclusion： Jiawei Buzhong Yiqi Decoction could relieve symptoms of AR through inhibiting SP release and SPR expression, decreasing the infiltrating density of mast cells and reducing the inflammatory reaction of tissues.