基于纳米钴信号放大化学发光生物传感器超灵敏检测DNA

Chemiluminescence Detection of DNA Hybridization Based on Cobalt Nanoparticles

研究了一种新颖简便的以大粒径（100 nm） Co纳米探针作为信号放大超灵敏化学发光检测DNA的新方法.该方法采用“三明治”夹心法,首先将壳核结构羧基化的纳米Co与氨基修饰的DNA结合制备纳米Co信号探针,然后通过靶DNA与固定了捕获探针的磁珠亲和形成一个“三明治”夹心的结构,制备纳米Co生物传感器.检测时,将纳米Co溶解成Co2＋,利用鲁米诺-H2O2-Co2＋化学发光体系对靶DNA进行检测,发现其对靶DNA的检测灵敏度可达到1.6×10-17mol·L-1（3δ）,其线性范围为0.6×10-16～4.0×10-16 mol·L-1。

A novel and sensitive flow injection chemiluminescence（CL） assay for the se quence specific DNA detection based on the signal amplication with cobalt nanoparticles （100 nm） was developed. The sandwich-type DNA biosensor was fabricated with the a- mino-functionalized capture DNA immobilized on the magnetic bead and target DNA was sandwiched between magnetic bead and a signal DNA probe labeled with cobalt NPs. The hybridization events were monitored by the Chemiluminescence （CL） intensity of luminol-H202-CO2＋ after the cobaltous ions were dissolved from the hybrids. The method could detect as low as 1.6 × 10 17 mol · L-1 target DNA and the line range was from 0.6 × 10 16 to 4.0 ×10 16 mol· L-1.