厌氧产甲烷反应器运行过程的三维荧光光谱的解析

Analysis of three-dimensional fluorescence spectra from anaerobic methane-producing process

结合平行因子法研究了厌氧产甲烷反应器的出水三维荧光光谱特征,研究结果表明,正常运行状态下反应器的荧光物质为蛋白质、辅酶NADH、辅酶F420。当反应器的底物浓度突然增加时,反应器出水的荧光光谱亦发生改变,辅酶F420荧光强度显著降低,反应器出水中的荧光组分以色氨酸、酪氨酸和辅酶NADH为主,表明产甲烷反应受到了抑制。平行因子法解析得到的荧光强度得分与荧光物质的相对浓度成正比,荧光物质种类和浓度的改变可以用来表征反应器运行的状况。因此,三维荧光光谱可以反映反应器处理过程中荧光物质种类和含量等信息,并可为污水处理过程有效监控提供快速分析方法。

The effluents from anaerobic methane -producing reactor were characterized by the EEM fluorescence spectra with PARAFAC methods .Results showed that the main components were in‐cluded of protein ,coenzyme NADH and F420 under normal operating conditions .After the influent concentration increased suddenly ,the EEM fluorescence spectra of the effluents changed correspond‐ingly .The fluorescence intensity of coenzyme F420 decreased substantially and the main fluorophores in the effluents was tryptophane ,tyrosine and coenzyme NADH ,which implicated that the anaerobic methane producing reaction was inhibited .The relative concentration of the fluorophores was correla‐ted with the fluorescence intensity scores .The changes of fluorophores in species and concentration could be used to reflect the operational status of the bioreactor .T herefore ,EEM fluorescence spectra could be used to acquire the information about the species and concentration of the fluorophores ,and they could provide a rapid and efficient method to monitor the wastewater treatment process .