荷斯坦成年奶牛枯草芽孢杆菌的分离与鉴定

Isolation and Identification of Bacillus subtilis Strains from Adult Holstein Cows

从健康荷斯坦成年奶牛新鲜粪便中分离纯化出1株疑似芽孢杆菌属菌株,编号为K。对该菌株进行形态学观察、生化试验、16SrDNA序列测定等。试验结果表明,该菌菌体革兰氏染色阳性,芽孢染色为绿色,呈长杆状,芽孢中生,椭圆;能发酵糖醇,吲哚试验阳性,触媒试验阳性,能产生淀粉酶,并可以液化明胶,16SrDNA测序序列同源性比较结果表明菌K为枯草芽孢杆菌;本试验为获得反刍动物有益菌种及研制反刍动物微生态制剂奠定基础。

One bacillus strain was isolated and purified from the faeces of healthy Holstein named K. Morphological observation, biochemical tests, 16S rDNA sequencing,etc, were conducted. Experimental results showed that strain K Gram stain was positive, spore staining was green. Its shape was a long rod--shaped bacteria, and spores was oval . Common sugar alcohols could be fermented; Indole test was positive; Catalyst test was positive; It could produce amylase; It also could liquefy gelatin. 16S rDNA sequence homology comparison results showed that the strain K was identified as Bacillus subtilis strain. So it provided theoretical basis for developing probiotic preparations in ruminats.