罗哌卡因与舒芬太尼对创面愈合的影响

目的研究罗哌卡因联合舒芬太尼对小鼠创面愈合的影响。方法健康sD小鼠128只，体质量20～25g，应用随机数字表法，将128只小鼠均分为4组（n=32）：空白生理盐水组（NS组），罗哌卡因组（L组），舒芬太尼组（S组），罗哌卡因＋舒芬太尼组（L＋S组）。于制造创面前10min，L和L＋S组皮下浸润0．5％罗哌卡因1．0mL／kg，S和L＋S组腹腔注射舒芬太尼3．0μg／kg稀释到10mL。分别于第3、7、10天每组取8只小鼠，观察创面愈合率，ELISA法测定GM－CSF的表达情况，每组另8只小鼠观察完全愈合时间。结果L＋S组愈合率3、7、10天分别为（39.394±1．164）％，（64．144±1.901）％，（83.543±1.120）％高于其他3组，愈合时间（10.250±0.463）d短于其他3组（P〈0．05），粒一巨噬细胞集落刺激因子（GM－CSF）的含量高于NS组和S组（P〈0.05），但与L组相比无统计学意义。结论皮下浸润0.5％罗哌卡因和腹腔注射舒芬太尼均可促进创面愈合，联合用药效果优于单独使用。

Objective To study the effect that ropivacaine combined with sufentanil on wound healing in mice. Methods There are 128 health of SD mice, 20-25 G body weight, according to the random number table, 128 mice were divided into 4 groups （n=32）： blank Group （NS group） and ropivacaine Group （L group）, sufentanil Group （S group）, ropivacaine with sufentanil Group （L＋S group）. Used in manufacture of 10 min,L＋S group were subcutaneous injected 0.5% ropicacaine by 1.0 mL/kg, S and L＋S groups were abdomainal injected sufentanil solution 1 mL, contains sufentanil 3 μg/kg. Choosing 8 mice in each group at 3, 7 and 10 days after the ops to check its wound healing rate and measure the expression of GM-CSF by the EL1SA method, the 8 left mice in each group were made to check its time of complete healing. Results The healing rate of L＋S group that is （39.394±1.164）%, （64.144±1.901）%, （83.543±1.120）% in the 3 th, 7 th and 10 th day of a manufacture wound respectively is higher than the left three groups and the healing time （10.250±0.463）d is also shorter than them （P〈0.05）, the expression of GM-CSF is higher than NS group and S group （P〈0.05）, but shows no statistical differences with L group. Conclusion Subcutaneous infiltration of 0.5% ropivacaine and intraperitoneal injection of sufentanil may promote the wound healing, combination therapy is better than the alone.