miR-126抑制结肠癌增殖及侵袭转移的功能研究

Suppression of proliferation and metastasis of colon cancer by miR-126

目的研究miR-126在体内是否具有抑制结肠癌细胞增殖及侵袭转移的功能。方法利用慢病毒载体构建稳定过表达miR-126的结肠癌细胞系HCT116,将实验组及对照组细胞分别进行裸鼠皮下及尾静脉成瘤体内实验。结果成功构建稳定过表达miR-126细胞系HCT116;裸鼠皮下成瘤实验结果显示过表达miR-126实验组皮下移植瘤瘤体平均体积明显小于其对照组瘤体体积（P〈0.05）;尾静脉成瘤组中稳定过表达miR-126实验组肺部转移灶的平均数目、面积均明显小于其对照组（P〈0.05）。结论裸鼠移植瘤实验证实miR-126在体内具有抑制结肠癌细胞增殖及侵袭转移的作用。

Objective To study the function of miR-126 in suppression of proliferation and metastasis of colon cancer in vivo. Methods We constructed stable overexpression of miR-126 colon cancer cell line HCT116 with lentiviral vector,and executed subcutaneous tumor formation and tail vein tumor formation test in nude mice with stable overexpression miR-126 colon cancer cell lines. Results Stable overexpression of miR-126 cell line HCT116 was successfully constructed. Nude mice tumor formation experiment results showed that the mean tumor volume of overexpression of miR-126 in experimental group was significantly less than the tumor volume in control group（ P〈0. 05）. Tail vein tumor formation experiment results showed that the average number and size of lung metastases of overexpression of miR-126 in experimental group was significantly less than those in control group. Conclusions Nude mice tumor formation experiment results confirm that miR-126 can inhibit colon cancer cell＇s proliferation and metastasis in vivo.