基于光动力疗法的吲哚菁绿体外血管内皮细胞光毒性

Photo-toxic effects of indocyanine green based on photodynamic therapy on vascular endothelial cells in vitro

目的:研究体外吲哚菁绿(ICG)介导的光动力疗法对血管内皮细胞的毒性作用。方法:人脐静脉内皮细胞以等量密度接种于96孔板中培养,按加入ICG的剂量不同分成0、50、100、150、200和250μmol/L组,共6组,每组8孔,其中4孔用波长为810 nm能量密度为24.0 J/cm2的半导体激光照射,另4孔不照射作对照。24 h后用MTT法测量各组细胞的存活率,并对细胞染色镜检。结果:在810 nm的激光照射下,含有ICG的各组内皮细胞的存活率较未照射组显著下降(均P<0),且随着ICG浓度的升高,光照组细胞存活率逐渐降低(P<0)。当ICG的剂量超过150μmol/L时,细胞的形态变化从凋亡为主转变为坏死为主。结论:适当剂量的ICG介导的光动力疗法对体外培养的血管内皮细胞有较强的抑制作用,有望成为新生血管性疾病的一种新的治疗药物。

Objective To investigate the photo-toxic effects of indocyanine green（ICG） based on photodynamic therapy（PDT） on vascular endothelial cells in vitro. Methods Human umbilical vein endothelial cells were incubated with ICG with different concentrations in 96-well plate. According to the dose of ICG, they were divided into 6 groups as 0, 50, 100, 150, 200 and 250 μmol / L group. Each group consists of 8 wells,4 of them were irradiated with a 810 nm laser of total power 24.0 J / cm2. Cell viability was measured by MTT assay 24 h after light irradiation. Morphological changes in cells were observed by light microscope. Results With the irradiation of 810 nm light, there was a significant decrease in relative cell viability in all groups except 0 μmol / L group（all P〈 0）. With the increase of concentration of ICG, the cell viability dropped rapidly（P 〈0）. Apoptotic changes can be seen in all groups except 0 μmol / L group and necrosis was the main change in cells of 200 μmol / L and 250 μmol / L group. Conclusion PDT with appropriate concentration of ICG has a strong inhibition to vascular endothelial cells in vitro and ICG-PDT may be a good new therapy for nevascularization.