方波电容耦合电场促进脊髓轴突再生

Axon regeneration of spinal cord with square wave capacitively coupling electrical field(SWCCEF)

应用自行设计的新型非置入式电场──方形波电容耦合电场和辣根过氧化酶（ＨＲＰ）标记技 术对脊髓轴突再生进行实验研究。选取２０只Ｓｐｒａｇｕｅ－Ｄａｕｌｅｙ大白鼠，体重２５０±１５９，在２０％乌拉坦腹腔 内麻醉下（１ｇ／ｋｇ），切除Ｔ９－１０椎板，将Ｔ１０脊髓半切断。将造模动物随机分为两组，每组１０只，Ⅰ组为方 波电容耦合电场治疗组，Ⅱ组为对照组，不作任何处理。４周后，沿动物原切口再切开，将２０％ＨＲＰ注入 Ｔ１０脊髓内５ｍｌ。动物存活３６ｈ，取出脑及脊髓，置入蔗糖缓冲液１２～２４ｈ，保持４℃，然后用冰冻连续 切片机切成４０μｍ厚度的切片，以ＴＭＢ为着色剂，通过在红核、尾状核、缝核及齿状核所标记的细胞核 团计数，发现两组之间有非常显著性差异（Ｐ＜０．０１）。结果提示方波电容耦合电场能促进大白鼠的脊髓轴突再生。

A new non-invasive form of electrical field-SWCCEF was designed in 1990, and have been engaged for experimental study. The observation of spinal cord axons regeneration by using Horseradish Peroxidase(HRP) label technique is reported in this paper. A total of 20 Sprague-- Dau-ley rats were used, weighing 250±15g, underwent lamineotomy at T9-10 under 20% Urethane(1g/ kg) being injected into abdominal cavity, and semiresection of T10 spinal cord was performed. The animals were equally allocated and into two groups. Group I ware treated with SWCCEF, group II untreated. After 4 weeks, the animals were reoperated along primary incisine and injected with 20%, HRP 5ml into T12 spinal cord. 36h later, the brain and spinal cord were removed, and placed in the sucrose buffer solution at 4℃ for 12 ~ 24h and then cut into 40 micra thick forzen sections, stained with TMB as the chromogen. The statistic analysis number of label cells counted of in red, caudate, raphe and dentate nucleus between the two groups was very significant difference (P< 0. 01). The result indicated that SWCCEF could promote spinal cord axons regeneration in rats.