摘要
目的 :探讨流式细胞术中不同分析软件在小鼠造血干细胞分析中的特点和差异。方法 :使用小鼠骨髓细胞悬液作为实验材料,在BD Aria III和BD Influx流式细胞仪上检测小鼠造血干细胞,分别应用FACSDiva、FACSSortware软件对检测数据进行分析,并比较分析结果。结果:FACSDiva和FACSSortware软件在参数调节、补偿调节、圈门、数据保存和分选设置方面存在细微差异,均可以实现多色流式分析和高速流式分选。结论:FACSDiva和FACSSortware软件分析同一样本所得结果有较高的一致性,均是使用流式细胞仪不可或缺的工具。
Objective To explore the characteristics and differences of kinds of flow cytometry operation software for mice bone marrow hematopoietic stem cell analysis. Methods With mice bone marrow cells as experimental materials, cells were stained by antibodies and measured by BD Aria III and BD Influx flow cytometer. The results of hematopoietic stem cell were analyzed and compared by FACSDiva and FACSSortware software. Results Discrepancies of FACSDiva and FACSSortware involved parameter regulation, compensation regulation, gating, data storage and sorting parameter setting.Both of the softwares were effective to complete multicolor flow analysis and high-speed cell sorting. Conclusion Results of the same sample analyzed by FACSDiva and FACSSortware can obtain high consistency.
出处
《医疗卫生装备》
CAS
2015年第3期5-8,共4页
Chinese Medical Equipment Journal
基金
国家自然科学基金资助项目(81001377)
