基于肾上腺切除+自体大网膜移植的雄激素全阻断大鼠模型的建立

Androgen deprivation rat modeling by removing adrenalines and autologous ectopic transplanting

目的建立基于肾上腺切除+自体大网膜移植的雄激素全阻断大鼠模型,为临床上实现真正意义上的雄激素全阻断治疗前列腺癌提供理论和实验依据。方法将32只成年雄性SD大鼠随机分为4组各8只,A组:双侧睾丸切除+双侧肾上腺切除+肾上腺皮质组织块自体大网膜移植;B组:双侧睾丸切除;C组:双侧肾上腺切除+肾上腺皮质组织块自体大网膜移植;D组:假手术组。术后7周检测肾上腺移植体、前列腺生长情况及大网膜(含肾上腺移植体)激素分泌水平。结果术后7周,A、C组大鼠肾上腺皮质组织块在大网膜上存活、再生,在组织形态学上有球状带、网状带、束状带细胞,但细胞排列较不规则。A组大鼠前列腺大体完全萎缩,组织学上腺泡明显萎缩,腔体皱缩,腺体分泌物减少,B、C组次之。A组和C组大网膜组织内的皮质醇、皮质酮及醛固酮水平均显著高于B组和D组(均P<0.001)。结论双侧睾丸切除+双侧肾上腺切除+肾上腺皮质自体大网膜移植既能相对彻底地阻断雄激素,又能最大限度地保留肾上腺皮质功能,在内分泌治疗晚期前列腺癌时具有一定的潜力。

Objective To establish androgen deprivation rat modeling by removing rat testicles and bilateral adrenalines and autologous ectopic transplanting so as to obtain scientific knowledge for treating prostate cancer. Methods 32 SA rats were randomly divided into four groups, namely, group A, B, C, and D. Treatment for group A included removal of testicles, removal of bilateral adrenal, and transplanting adrenal cortical tissue under autologous epiploon. Treatment for group B included removal rat testicles. Treatment for group C included removal of bilateral adrenalines and implanting adrenal cortical tissue under autologous epiploon. Treatment for group D included sham operation. Findings The prostate of group A atrophied evidently, greater omentum implant survived and regenerated with endocrine function. Conclusion Androgen deprivation by means of removal of testicles and bilateral adrenalines and autologous epiploon transplanting can make an effective method for treating prostate cancer.