非线性电化学指纹图谱鉴别党参与秦艽

Identification of Codonopsis Radix and Gentianae Macrophylae Radix by Nonlinear Electrochemistry Fingerprint

目的:建立党参与秦艽的非线性电化学鉴别方法。方法:在玻璃反应器中加入粒径小于0.125 mm的中草药粉一定量(准确到0.002 g)作为反应的底物,采用H+-Mn2+-CH3COCH3-Br O-3为振荡体系,控制温度310 K和搅拌速度500 r·min-1。以饱和甘汞电极作参比,铂电极作指示电极,采用电化学工作站记录化学振荡体系中电位E随时间t的变化,利用Origin 7.5软件绘制E-t曲线,获得非线性电化学指纹图谱。考察图谱的特征参数:最高电位、诱导时间、振荡寿命、振荡周期,计算反应的活化能,运用聚类分析法对其进行研究。结果:非线性电化学指纹图谱的重复性良好,直观上看党参与秦艽的非线性电化学指纹图谱具有明显的差异,特征参数也有明显的不同,聚类分析将样品分为两类。结论:可以利用此方法鉴别党参与秦艽。

Objective： To establish the method for identification of Codonopsis Radix and Gentianae Macrophylae Radix. Method： In a glass reactor with diameter less than 0. 125 mm of Chinese herbal medicine powder （ accurate to 0. 002 g） as the substrate of reaction, the H^＋-Mn^2＋-CH3COCH3-BrO3^- for oscillation system. Temperature kept at 310 K and the stirring speed was 310 r ·min^-1. With saturated calomel electrode as reference, the platinum electrode as indicator electrode, the electrochemical workstation records of chemical potential in the oscillation system, along with the change of time. Draw E-t curve using Origin 7.5 software, get the non-linear electrochemical fingerprint. The character of parameters： the highest potential, induction time, oscillation oscillation cycle and service life, calculate the activation energy of reaction, using cluster analysis method to study. Result： The repeatability is good, the non-linear electrochemical fingerprint intuitively see the party to participate in the non-linear electrochemical fingerprint has obvious difference, also have obvious different characteristic parameters, sample cluster analysis divided into two categories. Conclusion： This method can be used to identify Codonopsis Radix and Gentianae Macrophylae Radix.