滋肾清肝降糖方对2型糖尿病大鼠蛋白酪氨酸磷酸酶1B及氧化应激的影响

Effect of Zishen Qinggan Jiangtang Compound on PTP1B and Oxidative Stress in Type 2 Diabetic Rats

目的:研究滋肾清肝降糖方(ZQJC)对2型糖尿病(T2DM)大鼠糖代谢、蛋白酪氨酸磷酸1B(PTP1B)表达及超氧化物歧化酶(SOD)、丙二醛(MDA)和游离脂肪酸(FFA)的影响。方法:70只雄性SD大鼠随机抽取10只大鼠为正常组,其余大鼠采用高脂饲料喂养联合ip 30 mg·kg-1链脲佐菌素(STZ)的方法造T2DM模型,分为模型组、二甲双胍组(Met,0.14 g·kg-1·d-1,ig)、滋肾清肝降糖方低剂量组(0.9 g·kg-1·d-1,ig)、滋肾清肝降糖方中剂量组(1.8 g·kg-1·d-1,ig)及滋肾清肝降糖方高剂量组(3.6 g·kg-1·d-1,ig)。检测药物干预2,4周后各组大鼠空腹血糖(FBG)以及4周后各组大鼠血清中SOD,MDA,FFA的水平及肝细胞匀浆中PTP1B蛋白表达。结果:与正常组比较,模型组的FBG均升高(P<0.01),PTP1B在肝中的表达增加(P<0.01);与模型组比较,ZQJC各剂量组可显著降低大鼠血清FBG,FFA和MDA的含量(P<0.01,P<0.05),升高血清SOD的活性(P<0.05),ZQJC可降低PTP1B在肝中的表达。结论:滋肾清肝降糖方对2型糖尿病大鼠有调节糖代谢的作用,抑制PTP1B表达及氧化应激反应可能为其作用机制之一。

Objective： To study the effect of Zishen Qinggan Jiangtang compound （ZQJC） on glucose, protein tyrosine phosphatase 1B （PTP1B） expression, superexide dismutase （SOD）, malondialdehyde （MDA） and free fatty acide （FFA） in type 2 diabetic mellitus （T2DM） rats. Method： Ten rats were randomly selected as normal group, type 2 diabetes model was induced with high sucrose-fat diet and STZ ip for 30 mg·kg^-1, rats were randomly divided into groups： model group, metformin （Met） group （0. 14 g ·kg^-1·d^-1, ig）, ZQJCL group （0.9 g·kg^-1·d^-1, ig）, ZQJCM group （1.8 g·kg^-1·d^-1, ig） and ZQJCH group （3.6 g·kg^-1·d^-1, ig）, 10 rats in each group, fasting blood glucose （FBG） was detected at 2^rd week and 4^th week, and the level of SOD, MDA, FFA in the rats＇ serum and PTP1B expression in liver at 4^th week were measured after the treatment. Result： Compared with the normal group, the blood glucose levels of model group were increased （P 〈 0.01 ）, PTP1B expressions in liver of model group were increased （P 〈 0.01 ）. After treatment, compared with model group, ZQJC group could obviously decrease the levels of FBG, MDA and FFA （P 〈 0.05, P 〈 0.01 ）, improve SOD activity （P 〈 0.05） and inhibit the PTP1B expression in liver. Conclusion： ZQJC has the effect of regulating glucose metabolism of the T2DM rats, inhibition of oxidative stress and PTP1B expression in liver could be one of the mechanisms.