1,3-丁二烯作业工人遗传损伤与细胞周期蛋白D1基因多态性的关系

Association between CCND1 polymorphisms and chromosomal damage among workers exposed to 1,3-butadiene

目的探讨1,3-丁二烯（BD）作业工人外周血淋巴细胞遗传物质损伤状况及其与细胞周期蛋白D1（CCND1）基因多态性的关系。方法采用外周血淋巴细胞胞质分裂阻滞微核（CBMN）试验和彗星试验评价171名BD作业工人和55名非职业暴露对照人群的染色体和DNA损伤水平,应用聚合酶链反应-限制性片段长度多态性方法分析CCND1基因的多态性。同时,通过问卷调查的方式了解被调查者的年龄、性别、吸烟、饮酒和个人职业暴露史。结果 BD作业工人外周血淋巴细胞微核率和彗星尾距分别为（7.17±5.09）‰和4.65（95%CI 3.49-5.98）,均高于对照组（3.82±2.37）‰和2.38（95%CI 0.82-3.98）,差异有统计学意义（P〈0.01）;暴露组中具有CCND1基因A870G位点GG基因型个体的彗星尾距显著低于AA基因型个体（P〈0.05）。结论在现有BD暴露水平下,外周血淋巴细胞胞质分裂阻滞微核法和彗星试验均能检出BD作业工人的遗传物质损伤;CCND1等位基因A870G位点GG基因型可能与职业性BD暴露导致的外周血淋巴细胞DNA损伤水平增加风险相关联。

Objective To investigate the association between CCND1 polymorphisms and the DNA and chromosome damage in peripheral blood lymphocytes among workers exposed to 1,3-butadiene（ BD）. Methods 171 workers exposed to 1,3-butadiene and 55 controls without occupational BD exposure were investigated. Cytokinesis-block micronucleus（ CBMN） detection and comet assay were used to evaluate the chromosomal and DNA damage levels in peripheral blood lymphocyte. And the genotypes of CCND1 were detected by PCR-RFLP. Personal information including occupational history,age,sex,smoking and drinking status was collected by the questionnaire. Results The rate of CBMN and OTM of lymphocyte in BD exposed workers[（ 7. 17 ± 5. 09） ‰ and 4. 65（ 95%CI 3. 49- 5. 98）,respectively]were higher than those in controls[（ 3. 82 ± 2. 37） ‰ and2. 38（ 95% CI 0. 82- 3. 98）,P 〈0. 01]. Multivariate analysis of covariance revealed that in BD workers. The subjects with CCND1 A870 G AA allele have significantly higherrisk for DNA damage than subjects with GG allele（ P 〈0. 05）. Conclusion Under present BD exposure levels,both comet assay and cytokinesis-block micronucleus test could be sensitive group biomarkers to use to monitor the genotoxicity of BD occupational exposure. CCND1 A870 G polymorphisms might influence the susceptibility of DNA damage in BD exposed workers.