基于rDNA-ITS序列的天门冬拟茎点霉与相似种的系统发育关系

Phylogenetic relationships of Phomopsis asparagi and related speices based on internal transcribed space of ribosomal DNA( r DAN-ITS) sequences

芦笋茎枯病由天门冬拟茎点霉(Phomopsis asparagi)引起,为明确P.asparagi与寄生在其他蔬菜、水果和经济林木等植物上的拟茎点霉不同种之间的系统发育关系,对分离至福建漳州的P.asparagi菌株及从Gen Bank下载的相关菌株的35条ITS序列进行多序列比对分析,并用Neighbor-Joining法构建系统发育树。系统发育分析结果显示:35个菌株被划分为Ⅰ、Ⅱ和Ⅲ组群;P.asparagi与葡萄拟茎点霉(P.viticola)、叶下朱生拟茎点霉(P.phyllanthicola)、杨桃拟茎点霉(P.averrhoae)、大豆茎溃疡病菌(Diaporthe phaseolorum)聚在I组群的A亚群,它们的系统学关系最近;大豆拟茎点霉(P.longicolla)、大豆间座壳菌(Diaporthe sojae)、褐纹拟茎点霉(P.vexans)、光叶子花拟茎点霉(P.glabrae)、柑橘间座壳菌(Diaporthe citri)、黄瓜间座壳菌(Diaporthe sclerotioides)聚在Ⅱ组群,它们与P.asparagi的系统学关系较远;昏暗拟茎点霉(P.obscurans)单独聚在最远的分支上(Ⅲ组群),其与P.asparagi的系统学关系最远。

To define the phylogenetic relationships of Phomopsis asparagi and related speices that parasitized other vegetables,fruits and economic trees,35 internal transcribed space（ ITS） of ribosomal DNA（ r DNA-ITS） sequences of P. asparagi isolated from Zhangzhou city of Fujian province and the related speices downloaded from Gen Bank were together processed by multiple sequences alignment analysis,and the phylogenetic tree was constructed by Neighbor-Joining method.The Phomopsis strains were clustered into group I,group II and group III. P. asparagi,P. viticola,P. phyllanthicola,P.averrhoae and Diaporthe phaseolorum were clustered together in subgroup A of group I. They were closely related,but were relatively differentiated to some extent. P. longicolla,Diaporthe sojae,P. vexans,P. glabrae,Diaporthe citri and Diaporthe sclerotioides were clustered in group II. They had a distant relationship with P. asparagi. P. obscurans was clustered in the farthest distance clade（ group III）,indicative of the farthest phylogenetic relationship with P. asparagi.