外阴尖锐湿疣患者宫颈HPV的DNA分型检测及临床分析

Cervical HPV DNA genotyping and clinical analysis among patients with vulva condyloma acuminatum

目的:探讨外阴尖锐湿疣(CA)患者中宫颈人乳头瘤病毒(HPV)的DNA分型情况及与宫颈湿疣的发生、发展及癌变的相关性。方法:采用反向斑点杂交法对80例外阴CA患者宫颈HPV-DNA型(18种高危型和5种低危型)进行分型检测,用同期无外阴CA的80例宫颈HPV-DNA作对照。结果:外阴CA患者伴宫颈HPV阳性占61.3%,明显高于对照组的15.0%(P<0.01),两者都以单一低危型表达最多。外阴CA患者不同年龄段的HPV病例数比较差异无统计学意义(P>0.05),宫颈HPV多发于30岁以下患者;各年龄组HPV感染与尖锐湿疣之间均存在显著相关性(P<0.05)。结论:外阴尖锐湿疣患者宫颈HPV感染常见,应重视宫颈HPV的分型检测,及时发现高危人群,预防宫颈癌的发生。

Objective： To explore the correlation between cervical human papillomavirus （HPV） DNA genotyping among patients with vulva condyloma aeuminatum and the onset, development, and canceration of cervical condyloma acuminatum. Methods： Reverse dot blot hybridization technique was used for cervical HPV DNA genotyping （including 18 high risk genotypes and 5 low risk genotypes） among 80 patients with vulva condyloma acuminatum; 80 women without vulva condyloma acuminatum during the same time were selected as control group. Results： In vulva condyloma acuminatum group, 61.3% of the patients were found with positive cervical HPV, which was statistical- ly Significantiy higher than that in control group （ 15. 0% ） （P 〈0. 01 ） ; in the two groups, single low risk genotypes were the main types. In vulva condyloma acuminatum group, there was no statistically significant difference in the number of patients with HPV among the patients in different age groups （ P 〉 0. 05）, cervical HPV was mainly found among the patients under 30 years. In each age group, there was a significant correlation between HPV infection and condyloma acuminatum （ P 〈 0. 05 ） . Conclusion： Cervical HPV infection is common in patients with condyloma acuminatum, more attention should be paid to cervical HPV genotyping to timely find the high risk population and pre- vent cervical cancer.