40例MDS患者骨髓单个核细胞内自噬相关基因BECLIN-1表达水平和自噬泡数量检测及其意义

Expression of Autophagy Related Gene BECLIN-1 and Number of Autophagic Vacuoles in Bone Marrow Mononuclear Cells from 40 Myelodysplastic Syndromes Patients and Their Significance

目的:本研究旨在检测骨髓增生异常综合征(MDS)患者骨髓单个核细胞(BMMNC)内自噬相关基因BECLIN-1的表达水平和自噬泡数量,探讨M DS在不同阶段其骨髓单个核细胞内BECLIN-1表达水平和自噬泡数量的差异及其与疾病特点之间的关系。方法:收集9例正常对照、19例低危MDS、14例高危MDS以及7例MDS转化的急性髓系白血病(AML)患者的骨髓单个核细胞;采用实时定量PCR检测自噬相关基因BECLIN-1的表达,在透射电子显微镜下计数细胞内自噬泡数量。结果:低危组BECLIN-1表达水平明显高于正常对照(P<0.05);高危组BECLIN-1表达水平高于正常对照,但差异无统计学意义(P>0.05);转AML组BECLIN-1表达水平明显低于对照组(P<0.05)。电子显微镜结果显示,低危组和高危组自噬泡数量较对照组增多,但差异无统计学意义(P>0.05);转AML组自噬泡数量明显减少(P<0.05)。结论:在MDS疾病进展并向白血病转化的过程中患者骨髓单个核细胞内自噬相关基因BECLIN-1的表达水平逐渐降低,自噬泡数量逐渐减少;自噬可能与M DS的疾病进展相关。

Objective： The purpose of this study was to detect the expression level of autophagy related gene BECLIN-1 and the number of autophagic vacuoles in bone marrow mononuclear cells （BMMNC） from myelodysplastic syndrome（MDS ） patients and to explore their difference in different stage of MDS and relationship between their difference and disease characteristics. Methods： The BMMNC from 9 normal controls, 19 cases of low-risk MDS, 14 cases of high-risk MDS and 7 cases of MDS-transformed AML were collected. The expression level of BECLIN-1 was detected by real time PCR （RT-PCR） and the amount of autophagic vacuoles was counted by transmission electron microscopy. Results. The expression level of BECLIN-1 in BMMNC from patients with low-risk group was obviously higher than that in BMMNC from normal controls; the expression level of BECLIN-1 in BMMNC from patients of hgh risk group was higher than that in BMMNC of normal group, but there was no statistical significance （ P 〉 0.05 ） ; the expression level of BECLIN-1 in BMMNC from patients with MDS-transformed AML group was significanly lower than that in BMMNC of normal group （P 〈 0.05）. Transinission electron microscopy showed that the amount of autophagic vacuoles in BMMNC from patients with low-risk and high-risk MDS groups was more than that in normal control, but there was no stetistcal significance （ P 〉 0.05 ）, while the amount of autopuagic vecuoles in BMMNC from patients of MDS-transformed AML group was significantly less （ P 〈 0.05 ）. Conclusions： The expression of BECL1N-1 and the number of autophagic vacuoles in BMMNC from patients with MDS progression and patients with MDS-transformed AML are gradually declining. The autophagy may be associated with disease progression.