摘要
目的探讨乙二胺四乙酸（EDTA）治疗慢性镉中毒动物模型毒副作用。方法将雄性SD大鼠、雄性NIH小鼠分别随机分为空白对照组和模型组。模型组腹腔注射cdcl2＋β-巯基乙醇（ME）的混合液，每日一次，连续5d，末次染毒50d及30d后分别随机分为模型对照组和EDTA治疗组。空白对照组和模型对照组腹腔注射生理盐水；EDTA治疗组按体质量腹腔注射EDTA溶液，实验结束后均采集动物的尿液、血液肾脏及睾丸组织，检测体内Cd和组织病理检查。结果实验结束时，EDTA组大鼠、小鼠的肾脏Cd、尿Cd和血Cd含量明显高于模型对照组（P〈0．05），并见肾小管蛋白质沉淀。结论EDTA会引起体内的镉向肾脏富集，不宜用于慢性镉中毒驱镉。
Objective To investigate the side effects of （EDTA） utilized to treat chronic Cadmium poisoning in animals. Methods Male SD rats and NIH mice were randomly divided to the control group and model group respectively. The model group animals were daily given CdC12 ＋ 2-Mercaptoethanol （ME, ip） for 5 days. 50 days or 30 days later, the rats or mice were randomly divided into model control group and EDTA treatment group respectively. The control group and model control group were injected with saline, and the EDTA group animals were treated with EDTA solution .At the end of the experiment, the urine, blood, kidneys and testis were collected for detecting the Cd concentration and pathologic changes. Results Compared with the model control group, in the EDTA treated groups （both in rats and mice）, the Cd levels of kidney, urine and blood all increased significantly （P〈0.05）; protein precipitation could be detected in kidney tubules. Conclusion The EDTA could promote the accumulation of cadmium in the kidnev. EDTA is not recommended to treat chronic cadmium poisoning patients.
出处
《实验动物与比较医学》
CAS
2015年第1期56-60,共5页
Laboratory Animal and Comparative Medicine
基金
广东省重人科技专项（2012A080201011）,佛山市院市合作项目（2012A04）
