应用ClinProt技术筛查寻常性银屑病不同中医证型患者的相关蛋白表达特征

Applying Clin Prot Technology to Detect the Expression Characteristic of Proteins in Psoriatic Vulgaris with Different Syndrome Patterns

目的应用Clin Prot技术联合MALDI-TOF技术,检测正常人和不同中医证型寻常性银屑病患者血浆及血T淋巴细胞膜上的相关蛋白。方法选择血热证、血瘀证、血燥证的寻常性银屑病患者各10例,同时选取10例健康人作为对照组,采用ClinProt技术联合MALDI-TOF技术建立血浆和T淋巴细胞膜差异蛋白图谱进行对比。结果与对照组的T淋巴细胞膜蛋白相比,血热组有1个表达上调的差异蛋白峰(4 747D),血瘀组有1个表达上调(4 747D)、2个表达下调的差异蛋白峰(2 068D、7 764D),血燥组有1个表达上调的差异蛋白峰(4 747D)。而对照组和不同证型银屑病患者的血浆差异蛋白相比较,血热组有3个表达上调的差异蛋白峰(6 631D,9 288D,4 965D),血瘀组有5个表达上调的差异蛋白峰(2863D,4 418D,6 631D,4 965D,3 241D),血燥组有2个表达上调的差异蛋白峰(4 965D,9 288D)和1个表达下调的差异蛋白峰(3 957D)。结论不同证型寻常性银屑病患者与健康人相比,血T淋巴细胞膜上及血浆中存在差异蛋白峰,这些蛋白可能与各个证型寻常性银屑病的发病机制有关。

Objective To detect plasma proteins and T Lymphocytes membrane proteins in normal people and psoriasis vulgaris patients with different syndrome patterns by using ClinPrat combine with MALDI-TOF technologies. Methods Ten cases of blood-heat pattern, blood-stagnation pattern and blood-dryness psoriasis vulgaris pa- tients were enrolled, respectively. Meanwhile, 10 cases of healthy people were also recruited as control group. ClinProt combined with MALDI-TOF technologies were used to establish the different proteomic map of plasma protein and T lymphocytes membrane protein. Results Comparing with healthy group, one differ- ential T lymphocytes membrane protein up-regulated peak（4 747D） was noted in blood-heat group, along with one up-regulated peak （4 747D） and two down-regulated peaks （2 068D, 7 764D） in blood-stagnation group, and one up-regulated peak （4 747D） in blood-dryness group. Comparing control group with psoriasis patients with different patterns, 3 up-regulated differential plasma protein peak （6 631D, 9 288D ,4 965D） were noted in blood-heat group, along with 5 up-regulated peak （2 863D,4 418D ,6 631D ,4 965D,3 241D） in blood-stagnation group, 2 up-regulated peak （4 965D,9 288D） and 1 down-regulated peak （3 957D） in blood-dryness group. Conclusion Psoriasis vulgaris patients with different syndrome patterns have different T lymphoeytes membrane and plasma differential protein peaks compared with healthy people, and these pro- teins might have a relationship with the pathogenesis of psoriasis vulgaris with different syndrome patterns.