低温硝基苯降解菌的降解动力学研究

Study on Degradation Kinetics of Low-temperature Nitrobenzene Degrading Bacteria

[目的]研究一株低温硝基苯降解菌的降解动力学。[方法]对一株耐低温硝基苯降解菌进行研究,考察了其最适生长条件及在不同硝基苯初始浓度下的生长和降解情况,并进行降解动力学研究。[结果]当温度为15℃,p H为7,摇床转速为140 r/min,接种量为10%时,最适宜该菌株生长。该菌株培养48 h对200 mg/L硝基苯的好氧降解率达60.53%。当硝基苯初始浓度>100 mg/L时,该菌株的降解动力学符合Andrews抑制方程-非竞争性底物抑制模型。[结论]该研究可为硝基苯实际废水生化处理提供理论依据。

[Objective] The research aimed to study degradation kinetics of low-temperature nitrobenzene degrading bacteria. [Method] The optimal growth condition of low-temperature nitrobenzene degrading bacteria,and its growth and degradation situations under different initial concentrations of nitrobenzene were inspected,and its degradation kinetics was studied. [Result] Under the condition of temperature 15 ℃,p H 7,rotation speed 140 r / min and inoculum amount 10%,it was optimal for the bacteria growth. After cultivated for 48 h,degradation rate of 200 mg / L nitrobenzene by the bacteria reached 60. 53%. When initial nitrobenzene concentration was more than 100 mg / L,degradation kinetics of the bacteria corresponded to Andrews inhibition equation- non-competitive substrate inhibition model. [Conclusion] The research could provide theoretical basis for biological treatment of actual nitrobenzene wastewater.