紫叶狼尾草的组织培养与快速繁殖

Tissue Culture and Rapid Propagation of Pennisetum setaceum ‘Rubrum'

以紫叶狼尾草(Pennisetum setaceum‘Rubrum’)的茎尖或带腋芽的节间为外植体材料,进行了组培快繁技术的实验研究,建立了离体快繁技术体系。结果表明,以MS+6-BA 1.0 mg·L^(-1)+IBA 0.5 mg·L^(-1)为较适宜的外植体诱导丛生芽的培养基;以MS+6-BA 1.5 mg·L^(-1)+NAA 0.1 mg·L^(-1)+IBA 0.5 mg·L^(-1)为最佳继代增殖培养基,增殖系数达6.5;以1/2MS+NAA 0.5 mg·L^(-1)+IBA 0.5 mg·L^(-1)为最佳生根培养基,生根率100%;将生根组培苗移栽炼苗,30 d后成活率达98%以上。

The study used stem tips and internodes with axillary buds as the explants, conducted the research of tissue culture techniques in Pennisetum setaceum ＇Rubrum＇ propagation, and established the rapid propagation system in vitro. The results indicated that MS＋6-BA 1,0 mg·L-1＋IBA 0.5 mg·L-1 was the suitable medium for explants induction of cluster buds; MS＋6-BA 1.5 mg·L-1＋NAA 0.1 mg·L-1＋IBA 0.5 mg·L-1 was the optimizing medium for multipropagation, the multiplication coefficient could reach up to 6.5; 1/2MS＋NAA 0.5 mg·L-1＋I- BA 0.5 mg·L-1 was the best medium for rooting, the rooting rate could reach to 100%. After 30 days transplant- ed into greenhouse, the survival rate of young plants were over 98%.