摘要
以秀丽野海棠叶片为外植体诱导愈伤组织并分化出不定芽,得到再生植株,建立了组织培养和快速繁殖体系。结果表明:培养基MS+6-BA 1.0 mg·L^(-1)+2,4-D 0.3 mg·L^(-1)适用于叶片外植体愈伤组织诱导和分化,诱导率为100%,分化率为85.83%;MS+6-BA 1.0 mg·L^(-1)+IBA 0.3 mg·L^(-1)适用于继代增殖培养,平均增殖系数为7.38,且不定芽较粗壮;MS+NAA 0.5mg·L^(-1)+活性炭1 g·L^(-1)适用于生根培养,生根率达到100%;将生长良好的无菌植株进行移栽驯化,存活率达到90%。
In this paper, we studied in vitro culture of Bredia amoena by using leaf explants, including callus induction, adventitious shoot differentiation and plantlet regeneration. The results showed that the best medium for callus induction and adventitious shoot differentiation was MS＋6-BA 1.0 mg·L-1＋2,4-D 0.3 mg.Ll, with an induction rate of 100% and a differentiation rate of 85.83%. The best multiplication medium was MS＋6-BA 1.0 mg·L-1＋IBA 0.3 mg·L-1, the multiplication coefficient reached 7.38, and the shoots induced were strong. The optimum rooting medium was MS＋NAA 0.5 mg·L-1＋activated carbon （AC） 1 g·L-1, and the rooting rate reached 100%. The strong rooting plantlets were transplanted into greenhouse and the survival rate was 90%.
出处
《植物生理学报》
CAS
CSCD
北大核心
2015年第2期241-245,共5页
Plant Physiology Journal
基金
浙江省科技计划项目(2013C32102)
浙江省观赏花卉工程技术研究中心(2012E0036)
丽水市科技计划项目(20120310)
关键词
秀丽野海棠
叶片
离体培养
植株再生
Bredia amoena
leaf blade
in vitro culture
plantlet regeneration
