摘要
目的借助于X-射线、CT扫描成像、MRI等医学成像技术评价中成药伤科接骨片对大鼠骨折模型的治疗作用。方法大鼠麻醉后手术分离右前肢桡骨,于中段形成横断的完全骨折,随后随机分为模型组、药物治疗组,另设伪手术组进行平行对照。治疗组按0.33g/kg的剂量灌胃给药,模型组和伪手术组等量灌胃生理盐水,连续给药4周,给药结束后以X射线、CT和MRI图像观察和记录骨折部位的愈合情况。最后麻醉处死动物,经解剖取骨折部位进行抗折力测定和HE染色的病理组织学检查。结果 X射线、CT和MRI检查结果清楚地表明,给药治疗后骨折部位可见有明显的致密性骨痂形成,骨折线模糊不清或消失,多数可见大量的钙盐沉积,趋于愈合,与骨折部位的病理组织学检查结果基本一致。结论借助于医学影像技术可以更加客观地评价药物对大鼠骨折模型的治疗作用,尤以CT四维成像技术更加直观、清晰,值得进一步地推广应用。愈合后的前肢桡骨的抗折力也明显地增强。
Objective To evaluate the therapeutic effect of a Chinese medicine, Shangke Jiegu Tablet, on the rat model of bone fracture. Methods After anesthesia, complete transverse fracture of the middle portion of the radius in the right forelimb was made in rats. The rats were randomly divided into three groups: model group, treatment group and sham operation group as control. Each group included 20 rats, half male and female. The rats in the treatment group were intra- gastrieally administered the drug in a dose of 0.33 g/kg once daily for 4 weeks, while the model and sham operation groups were given normal saline. After the end of drug administration, images of X-ray, CT and MRI were taken to record the healing of the bone fracture. Finally the rats were sacrificed by anesthesia, and the bones were taken for anti-rupture strength test and routine histopathological examination. Results The imaging examinations clearly showed dense callus for- mation at the fracture site, and the fracture line was blur or disappeared. Calcium deposition was observed in most fracture sites. It was consistent with the results of histopathological observation. The anti-rupture strength of the healed bones was also obviously increased in the treatment group. Conclusions Medical imaging techniques can be used to more objectively evaluate the therapeutic effects of drug therapy for bone fracture. Especially, the 4-dimensional CT imaging can more clear- ly and directly reveal the healing process of bone fracture, and is worth of popularization and application.
出处
《中国实验动物学报》
CAS
CSCD
北大核心
2015年第1期91-96,共6页
Acta Laboratorium Animalis Scientia Sinica
基金
江苏省科技公益专项基金资助(编号:BM2007506)
关键词
伤科接骨片
骨折
大鼠模型
影像学
Shangke Jiegu Tablet
Bone fracture
Rat model
Medical imaging
Histopathology
Therapy