氟中毒对小鼠心肌组织ATP酶、琥珀酸脱氢酶和总抗氧化能力的影响

Effect on ATPase,succinate dehydrogenase and total antioxidant capacity in mice myocardium of fluorosis

目的探讨不同浓度氟化钠对小鼠心肌组织ATP酶、琥珀酸脱氢酶和总抗氧化能力的影响。方法随机挑选40只昆明小鼠,建立动态染氟模型。分别在90、120 d测定不同浓度染氟后小鼠心肌组织中Ca2+-Mg2+-ATP酶、Na+-K+-ATP酶和琥珀酸脱氢酶(SDH)活力及总抗氧化能力(T-AOC)。结果与对照组相比,过量的氟会导致小鼠心肌组织Ca2+-Mg2+-ATP酶、Na+-K+-ATP酶和SDH活力及T-AOC显著降低,各组数据差异具有统计学意义。结论氟大量蓄积引起脂质过氧化作用增强,抗氧化酶活力受到抑制,使机体处于氧化应激状态;氟中毒导致体内脂质过氧化物大量蓄积,破坏了线粒体和细胞膜通透性,导致SDH和ATP酶活性下降。

Objective To evaluate effect of sodium fluoride of different concentration on ATPase,succinate dehydrogenase（ SDH） and total antioxidant capacity（ T- AOC） in mice myocardium. Methods Forty Kunming mice were randomly selected and exposed to a series of doses of sodium fluoride to establish a dynamic model. The myocardium homogenates were prepared to detect the activity of Ca2 ＋- Mg2 ＋- ATPase,Na＋- K＋- ATPase and succinate dehydrogenase（ SDH）,the content of total antioxidant capacity（ T- AOC）after 90 days and 120 days. Results Compared with the control group,the activity of Ca2 ＋- Mg2 ＋- ATPase,Na＋- K＋- ATPase,succinate dehydrogenase（ SDH） and total antioxidant capacity（ T- AOC） significantly decreased after excessive fluorosis exposure,there are statistically significant differences among each group. Conclusion The accumulation of fluoride in large quantities in mice resulted in the enhanced lipid peroxidation and the suppressed antioxidant capacity and made the mice to be in a state of oxidative stress; Fluorosis resulted in plenty of lipid peroxides in the mice and damaged mitochondria and cell membrane permeability,finally resulted in the activity of succinate dehydrogenase（ SDH） and ATPase decreased.