食管鳞癌肿瘤出芽与D2-40标记淋巴管浸润及淋巴结转移的相关性

Relationship among tumor budding, D2-40 monoclonal antibody-labeled lymphatic vessel invasion and lymph node metastasis in esophageal squamous cell carcinoma

目的:探讨食管鳞癌中肿瘤出芽与D2-40标记淋巴管浸润(lymphatic vessel invasion,LVI)及淋巴结转移的相关关系.方法:参照Ueno标准,通过HE染色观察并判断70例食管鳞癌肿瘤出芽情况,采用免疫组织化学(SP法)技术检测D2-40表达并观察肿瘤细胞浸润淋巴管情况,分析肿瘤出芽、LVI与临床病理指标间的关系.结果:70例食管鳞癌的高级别肿瘤出芽率和LVI阳性率分别为30%和40%,二者均与淋巴结转移、临床分期呈正相关(P<0.01).LVI与肿瘤分化程度呈负相关(P<0.05).肿瘤出芽与LVI符合率为72.86%.结论:联合检测肿瘤出芽和LVI可作为食管鳞癌判断淋巴结转移的预测因子.

AIM： To investigate the relationship among tumor budding, D2-40 monoclonal antibodyl abeled lymphatic vessel invasion（ LVI） and lymph node metastasis in esophageal squamous cell carcinoma（ESCC）. METHODS： According to the Ueno criteria, we assessed tumor budding by HE staining and examined the expression of D2-40 and LVI by immunohistochemistry in 70 ESCC cases. RESULTS： The positive rates of high-grade tumor budding and LVI were 30% and 40%, respectively. Both tumor budding and LVI was positively correlated with lymph node metastasis and clinical stage（P 〈 0.01）. LVI was negatively correlated with tumor differentiation（P 〈 0.05）. The coincidence rate between tumor budding and LVI was 72.86%. CONCLUSION： The joint detection of tumor budding and LVI will be useful for forecasting lymph node metastasis in ESCC.