银杏叶片治疗特发性肺间质纤维化临床研究和作用机制初探

Therapeutic Effect and Mechanism of Ginkgo Leaf Tablets for Idiopathic Pulmonary Fibrosis

目的:观察银杏叶片治疗特发性肺间质纤维化的临床效果,并探讨其相关的作用机制。方法:选取60例特发性肺间质纤维化患者,依据随机数字表法分为对照组和治疗组各30例。对照组给予常规治疗,治疗组在常规治疗的基础上加服银杏叶片,疗程为12周,比较2组的疗效。采用酶联免疫吸附法(ELISA)分别检测2组患者治疗前后血清中的转化生长因子-β1(TGF-β1)、肿瘤坏死因子-α(TNF-α)和基质金属蛋白酶-9(MMP-9)的表达水平。结果:对照组总有效率为60.00%,治疗组总有效率为83.33%,2组比较,差异有统计学意义(P<0.05)。治疗后,2组TGF-β1、TNF-α和MMP-9的表达水平均较治疗前下降(P<0.05,P<0.01);与对照组相比,治疗组3项细胞因子的表达水平均下降,差异均有统计学意义(P<0.05)。结论:银杏叶片治疗特发性肺间质纤维化具有良好的疗效,其作用机理可能与下调TGF-β1、TNF-α和MMP-9表达水平有关。

Objective： To observe the therapeutic effect of Ginkgo leaf tablets for the treatment of idiopathic pulmonary fibrosis,and to explore the relevant mechanism. Methods： Sixty cases with idiopathic pulmonary fibrosis were randomly divided into control group and treatment group,30 cases in each group. The control group was given conventional treatment,and the treatment group was treated with Ginkgo leaf tablets based on the conventional treatment. The treatment covered 12 weeks. Enzyme-linked immunosorbent assay（ELISA） was applied to detect serum transforming growth factor- β1（TGF-β1）, tumor necrosis factor alpha（TNF-α） and matrix metalloproteinases（MMPs） expression levels in the two groups. Results：The total effective rate was 83.33% in the treatment group,and was 60.00% in the control group, the difference being significant（P 0.05）. The levels of TGF-β1, TNF-α and MMP-9 were decreased after treatment in both groups（P 0.05 or P 0.01 compared with those before treatment）,and the decreasein the treatment group was superior to that in the control group（P 0.05）. Conclusion：Ginkgo leaf tablets are effective for idiopathic pulmonary fibrosis,and its relavant mechanism may be involved in decreasing the expression levels of TGF-β1,TNF-α and MMP-9.