山西省运城市2012年蚊媒病毒的分离鉴定

Isolation and identification of mosquito-borne arboviruses in Yuncheng city, Shanxi province, 2012

目的了解山西省运城市蚊虫及蚊媒病毒的种类和分布。方法2012年8月在山西省运城市临猗县和永济市采集蚊虫标本，经鉴定分类和分批研磨后，利用细胞（C6／36和BHK21）培养方法分离病毒，对阳性分离物使用蚊媒病毒种属特异引物进行RT-PCR扩增鉴定。结果采集4属7种10455只蚊虫，临猗县和永济市优势蚊种分别为淡色库蚊（91．96％）和三带喙库蚊（72．85％）。经鉴定分析，从标本中分离到15株基因I型乙型脑炎病毒（JEV）、4株库蚊黄病毒（CxFV）、3株淡色库蚊浓核病毒（CppDNV）和1株盖塔病毒（GETV）。结论首次从山西省分离GETV和CxFV；三带喙库蚊仍是运城市优势蚊种，目前当地自然循环的JEV仍以基因I型为主。

Objective To investigate the species and distribution of mosquitoes and mosquito-borne arboviruses in Yuncheng city of Shanxi province, China. Methods Mosquito samples were collected in 19 collection sites from Linyi county and Yongji city in Yuncheng city, in August, 2012. After identification and classification, all the specimens were homogenized and centrifuged to acquire supernatant before being inoculated to both C6/36 and BHK21 cells for viral isolation. Positive isolates were identified with arbovirus species-specific primers under RT-PCR, for further sequencing and phylogenetic analysis. Results A total of 10 455 mosquitoes of 7 species in 4 genuese were collected. The predominant mosquito species in Linyi county was Culex pipens pallens （91.96%,3 911/4 253） ,but the one in Yongji city was Culex tritaeniorhynchus （72.85%,4 518/6 202）. A total of 23 strains of viruses were isolated from the mosquito pools. 15 strains from Culex tritaeniorhynchus and Culex pipens pallens were identified as genotype I Japanese encephalitis virus （JEV）. Four strains from Culex pipens pallens were identified as Culex fiavivirus （CxFV）. Three strains from Culex pipens pallens were identified as Culex pipiens pallens densovirus （CppDNV）. One strain from Armigeres subalbatus and Aedes albopictus was identified as Getah virus （GETV）. Conclusion Four kinds of arboviruses were isolated from the mosquito pools, including GETV and CxFV, which were isolated and documented in Shanxi province for the first time. In the city of Yuncheng, Culex tritaeniorhynchus had been the predominant species and major vector for transmitting JEV. Genotype I JEV remained the major JEV circulating in the local natural environment.