摘要
目的:本研究分析激活的MEK/ERK信号通路在双歧杆菌脂磷壁酸促进小鼠腹腔巨噬细胞肿瘤坏死因子-α(TNF-α)增强表达中参与的机制。方法 :分离小鼠腹腔巨噬细胞并随机分为3组:对照组、脂磷壁酸组和脂磷壁酸+PD98059组(n=6)。酶联免疫吸附测定法检测各组巨噬细胞中TNF-α的含量,Western Blotting检测各组巨噬细胞中MEK/ERK信号通路的关键蛋白Ras、Raf、pMEK/MEK、pERK1/2及ERK1/2的表达。结果:与对照组相比,脂磷壁酸组小鼠巨噬细胞的TNF-α水平明显增高,差异具有统计学意义(P<0.01);MEK/ERK信号通路相关蛋白Ras、Raf、pMEK/MEK、pERK1/2及ERK1/2的表达明显上调,差异具有统计学意义(P<0.01),而PD98059可以明显抑制上述蛋白的异常(P<0.01)。结论:双歧杆菌脂磷壁酸通过激活小鼠腹腔巨噬细胞MEK/ERK通路促进TNF-α的上调表达。
Objective:To investigate the mechanism of TNFαup-regulation in mice peritoneal macrophage induced by lipoteichoic acid from Bifidobacterium.Methods:Mice peritoneal macrophage were divided in to 3groups:control,lipoteichoic acid and PD98059 group.The content of TNFαin each group was detected by ELISA and the expression of MEK/ERK signaling pathway proteins were assayed by Western Blotting.Results:The result showed that the content of TNFαwas increased greatly(P〈0.01)in lipoteichoic acid group while PD98059 can decrease it greatly(P〈0.01).The result also showed that the MEK/ERK pathway proteins Ras,Raf,pMEK/MEK,pERK1/2as well as ERK1/2 were up-regulated dramatically(P〈0.01).PD98059 also signified effectiveness in inhibiting it(P〈0.01).Conclusion:Lipoteichoic acid from Bifidobacterium enhances the expression of TNF-αby activating MEK/ERK signaling pathway in mice peritoneal macrophage.
出处
《海南医学院学报》
CAS
2015年第4期442-444,448,共4页
Journal of Hainan Medical University
基金
2012深圳科技计划项目(201202125)~~