CML患者循环内皮细胞的分子标志物与其体外增殖能力相关

Relationship of the markers and proliferation in vitro of circulating endothelial cells from chronic myelogenous leukemia patients

目的探讨慢性粒细胞白血病(CML)患者循环内皮细胞(CECs)的分子标志物与其体外增殖能力的关系。方法免疫磁珠分离CML患者的CECs,与同期培养的HUVECs进行生物学特性比较。用FISH检测CECs上bcr/abl融合基因的表达。结果 CML患者的CECs与HUVECs均有典型的血管内皮细胞形态,两者均高表达CD146及VWF,与HUVEC相比,CECs高表达CD34、KDR及CD133(P<0.05),增殖能力更强。12名CML患者CECs中bcr/abl阳性率为10.77%,与疾病进程呈正相关的趋势。结论证实CML患者的CECs不同于成熟内皮细胞,它们的高增殖能力可能与表达肿瘤特异融合基因有关。

Objective To investigates the relationship between molecular markers and proliferation in vitro of CECs from CML patients. Methods CECs were sorted by MACS and cultured to be compared with HUVECs in biology feature. We also examined expression of bcr / abl fusion gene by FISH in 12 CML patients. Results The cultured CECs and HUVECs had typical cobble-stone morphology; they could be identified by high level of CD146 and VWF. CECs derived from CML patients had higher level of CD34,KDR and CD133,and showed more increased proliferative potential as compared with HUVECs（ P 0. 05）. In 12 CML patients,the positive rate of bcr / abl fusion gene in CECs was 10. 77%. Higher expressions of bcr / abl fusion gene had positive correlation trend with progression of disease. Conclusions CECs in patients with CML are different from mature endothelial cells,they have increased proliferation by carrying bcr / abl fusion gene.