丰富环境对颅脑外伤大鼠学习记忆及海马神经元凋亡的影响

Effects of enriched environment on the function of learning,memory and apoptosis in hippocampus after traumatic brain injury in rats

目的:研究丰富环境对颅脑外伤大鼠学习记忆能力及海马神经元凋亡的影响。方法:32只SD大鼠采用自由落体法建立颅脑外伤模型,排除差异后纳入研究,分为丰富环境组和对照组。在造模后11天开始进行连续4天水迷宫检测大鼠学习记忆能力,之后取脑观察海马区神经元形态及使用TUNEL法检测海马区神经元凋亡水平。结果:在水迷宫检测第4天丰富环境组大鼠学习记忆能力较对照组大鼠明显改善,差异有显著性意义(P<0.05)。形态学检测发现丰富环境组大鼠海马区神经元凋亡水平较对照组明显减少,差异有显著性意义(P<0.05)。结论:丰富环境可明显改善颅脑外伤大鼠学习记忆能力,其机制可能与减少海马区神经元凋亡,提高神经元存活水平相关。

Objective：To examine the effects of enriched environment on improving the function of learning and memory,and increase the survival of neuron after traumatic brain injury in adult rats.Method：Thirty-two adult SD rats of traumatic brain injury model were randomly divided into standard environment group（SE,n=10）,and enriched environment group（EE, n=22）.After 11 days,all the rats received the Morris water maze test for consecutive 4d. The brain tissue will be extracted for immunohistochemical test（Nissle and TUNEL staining） when the Morris water maze tests finished.Result：The result of Morris water maze test showed that in both the navigation and space exploration experiments,EE group performed significantly better than SE group（P0.01）.The number of apoptotic cells declined markedly compared to SE group.Conclusion：EE interventions can reduce pathological damages in hippocampus following TBI, promote brain injury recovery,and also can enhance abilities of learning and memory.