成人嗅黏膜嗅鞘细胞的体外培养及迁移特性

The cultivation and migration in vitro of olfactory ensheathing cells from human olfactory mucosa

目的:探讨成人嗅黏膜嗅鞘细胞的分离、培养及体外迁移特性。方法:分离并培养成人及大鼠鼻腔嗅黏膜来源的嗅鞘细胞,分别观察2组嗅鞘细胞的生长、分型以及体外迁移能力。结果:成功分离培养成人和大鼠嗅黏膜嗅鞘细胞,成人嗅黏膜嗅鞘细胞的生长、分型、体外迁移能力及迁移特点与大鼠黏膜嗅鞘细胞相比无明显差异。结论:成人嗅黏膜嗅鞘细胞与大鼠嗅黏膜嗅鞘细胞相比,具有类似的细胞特性,可以作为临床细胞移植修复神经损伤的可靠的细胞来源。

Objective：To investigate the bionomics of the olfactory ensheathing cells（OECs）of human olfactory mucosa.Method：To separate and cultivate the OECs of human and rat olfactory mucosa.To observe the cell growth,cell grouping and cell migration in vitro of the two types of OECs.Result：Successfully separated and cultivated the OECs of human and rat olfactory mucosa.OECs of the human and rat olfactory mucosa had the similar cell growth,cell grouping and cell migration ability in vitro.Conclusion：OECs of the human and rat olfactory mucosa have the similar bionomics in vitro,as a result,OECs of the human olfactory mucosa could be a reliable source of cell transplant for nerve injury.