沈阳市2011～2013年麻疹实验室网络运转状况分析

Analysis on running status of Shenyang municipal measles laboratory network during 2011-2013

目的评价2011-2013年沈阳市麻疹实验室网络运转状况。方法回顾性分析2011-2013年沈阳市麻疹实验室网络检测结果及麻疹监测系统数据,分析麻疹病毒血清学检测、病毒分离与基因分型,以及健康人群抗体监测结果,并对实验室网络质量控制进行评价。结果 2011-2013年沈阳市麻疹监测系统报告麻疹疑似病例588例,确诊531例,其中实验室确诊麻疹275例（51.79%）;共采集血清标本515份,标本采集率为87.59%,其中采集合格并有实验结果的标本504份,标本检测率为97.86%。2011-2013年共采集麻疹病毒分离标本111份,麻疹病毒分离率为37.84%,且34株麻疹代表毒株基因型均为H1a型。2011-2013年沈阳市麻疹实验室通过了辽宁省麻疹实验室组织的职能考核和血清复核,且于2013年通过辽宁省麻疹实验室的现场考核。健康人群麻疹IgG抗体阳性率95.24%,保护率为78.10%,初免阳性率为94.12%,几何平均滴度（GMT）为1∶2 269.86。结论2011-2013年沈阳市麻疹实验室网络运转良好,在麻疹监测中起到了重要作用,应继续规范实验操作,加强实验室网络管理。

Objective To evaluate the running status of the Shenyang municipal measles laboratory network during 2011-2013.Methods The detection results of the measles laboratory network and the data of the measles surveillance system during 2011-2013 were retrospectively analyzed.The detection results of measles viral serological detection,viral isolation,genotyping and antibody monitoring results in the healthy population were analyzed and the quality control of the laboratory network was evaluated.Results 588 cases of suspected measles were reported by the Shenyang municipal measles monitoring system,515 cases were definitely diagnosed,in which 275 cases（51.79%）were diagnosed by laboratory;515serum samples were collected during 2011-2013,the collection rate was 87.59%,in which 504 samples were qualified and had the laboratory results,the sample detection rate was97.86%.111 samples of measles virus isolation were collected during 2011-2013,the measles viral isolation rate was37.84%.34 isolated strains of measles virus were verified as H1 agenotype.The Shenyang municipal measles laboratory passed the proficiency test and the serum samples recheck organized by the Liaoning provincial measles laboratory during 2011-2013,moreover passed the on-spot examination by the Liaoning provincial measles laboratory.The measles IgG antibody positive rate in the healthy population was 95.24%,the protective rate was 78.10%,the positive rate of first immune was 94.12%,the geometric mean titer（GMT）was 1∶2 269.86.Conclusion The running status of the Shenyang measles laboratory network is well during 2011-2013.It could play an important role in measles monitoring.The laboratory operation should be continued to normalized and the laboratory network management should be strengthened.