摘要
目的制定全自动血细胞分析仪显微镜复检规则,根据本实验室所用血细胞分析仪的特性,建立适合本科室血细胞分析仪复检的标准。方法用Beckman-Coulter LH750全自动血细胞分析仪和显微镜,对1 050份标本结果进行分析比对。结果需要复检的标本有229份,真阳性标本148份,真阳性率为14.1%,假阳性标本为81份,假阳性率为7.7%。其中真阴性标本为794份,真阴性率为75.6%;假阴性标本27份,假阴性率为2.6%。符合国际血液学复审组专家认为假阴性率<5.0%是保证患者检测结果可靠的最大可接受范围,复检率为21.8%。不同类别的报警提示分组中,细胞形态结果异常的假阳性率最高,达45.7%。对于仪器有这些警示项时,应涂片镜检。结论本科室制定的血细胞分析仪复检规则及标准符合要求。提高了血常规检验结果的准确性,从而减少或避免误诊、漏诊的发生。
Objective To enact the review rules of hematology analyzer,establish the suitable standard according to the characteristics of the hematology analyzer for our laboratory. Methods Using Beckman-Coulter LH750 hematology analyzer and microscopy,the detection results of 1 050 cases of blood samples were analyzed and compared. Results It showed that the results of 229 samples need to be reviewed,the real positive rate was 14. 1%( 148 samples),the false positive rate was 7. 7%( 81samples),the real negative rate was 75. 6%( 794 samples),and the false negative rate was 2. 6%( 27 samples). The false negative rate( 2. 6%) of our laboratory was in line with the false negative rate(〈5. 0%) formulated by International Hematology Review Group. The review rate was 21. 8%. Among different classes of alarm packet,the false positive rate of Abnormal cell morphology was the highest,up to 45. 7%. When these warnings of the instrument occur,microscopic examination should be operated. Conclu sion Our review rules and standards come up to the requirements. It improves the accuracy of routine blood test,diminishes or avoids the misdiagnosis and missed diagnosis.
出处
《中国卫生检验杂志》
CAS
2015年第5期691-693,共3页
Chinese Journal of Health Laboratory Technology
关键词
全自动血细胞分析仪
显微镜
复检规则及标准
Fully automatic hematology analyzer
Microscope
Review rules and standards