摘要
目的探讨无精子症和严重少精子症与遗传因素的关系。方法对69例无精子症和严重少精子症患者进行外周血染色体G显带核型分析,运用多重PCR技术检测Y染色体AZF基因家族AZFa、AZFb、AZFc三个区域中的6个序列标签位点(sequencet agged sites,STS)微缺失。结果 69例无精子症和严重少精子症患者中有染色体异常12例,Y染色体微缺失14例,其中有4例患者既存在染色体异常,又存在Y染色体微缺失。结论无精子症和严重少精子症与遗传缺陷密切相关。
Objective:To investigate the relationship between azoospermia and severe oligozoospermia and geneticfactors. Methods:69 cases of azoospermia and severe oligozoospermia patients peripheral blood chromosome G banding karyotype analysis,using 6 sequence tagged site detection of Y chromosome AZF gene family,AZFa,AZFb,AZFc multiple PCR technology in the three zone(sequencet aged sites,STS)microdeletion. Results:69 cases of azoospermia and severe oligozoospermia patients with chromosome abnormality in 12 cases,Y chromosome microdeletion in 14 cases,including 4 cases of both chromosomal abnormalities,and the presence of Y chromosome microdeletions. Conclusion: azoospermia and severe oligospermia and genetic defects are closely related.
出处
《中国优生与遗传杂志》
2015年第2期44-45,74,共3页
Chinese Journal of Birth Health & Heredity