右美托咪啶对脂多糖致伤大鼠肾小管上皮细胞Toll样受体4表达的影响

Influence of dexmedetomidine on Toll-like receptor 4 in renal tubular epithelial cells of rat injured by lipopolysaccharide

目的观察右美托咪啶对脂多糖致伤大鼠肾小管上皮细胞Toll样受体4(Toll-like receptor 4,TLR4)表达的影响,并探讨其可能的机制。方法购买大鼠肾小管上皮细胞,经复苏、培养,均加入10μg/L脂多糖,均经培养后分为3组。A组为空白对照组;B组为阳性对照组,加入右美托咪啶2.5μg;C组作为实验组,先加入阿替美唑10μg,30 min后加入右美托咪啶2.5μg。继续培养12 h。采取免疫组织化学法检测3组细胞TLR4表达及TLR4mRNA水平,并测定3组细胞凋亡率。结果B组应用右美托咪啶后,TLR4、TLR4mRNA和细胞凋亡率均低于A组(P<0.01);C组TLR4、TLR4mRNA和细胞凋亡率均高于B组(P<0.01)。结论右美托咪啶可下调脂多糖致伤大鼠肾小管上皮细胞TLR4表达,且右美托咪啶对TLR4的调节与α_2肾上腺素能受体被激动相关。

Objective To observe the effect of dexmedetomidine for Toll-like receptor 4（TLR4） in renal tubular epithelial cells on rat injured by lipopolysaccharide（LPS）,then to explore the possible mechanism.Methods Rat renal tubular epithelial cells were purchased,recovered,cultured,then added LPS 10 μg/L and were cultured and divided into three groups,group A,the blank control group,group B added dexmedetomidine 2.5 μg as control group,group C joined atipamezole 10 μg and added dexmedetomidine 2.5 μg after 30 min as the experimental group,all continued culture for 12 h.Then,immunohistochemistry was used to measure TLR4 protein expression and TLR4 mRNA in three groups,and the apoptosis rate of three groups were determined.Results After using dexmedetomidine,TLR4,TLR4 mRNA and apoptotic rate of group B were lower than those of A group（all P〈0.01）,while TLR4,TLR4 mRNA and apoptosis rate of group C were higher than those of group B（all P〈0.01）.Conclusion Dexmedetomidine can downregulate TLR4 expression in renal tubular epithelial cells of the rat injured by LPS,and the regulation for TLR4 of dexmedetomidine is related with o^adrenoglc recptor excited.