1764例血培养病原菌分布及耐药性分析

Distribution and drug resistance of pathogenic bacteria in 1 764 blood samples by culture

目的了解血培养中常见病原菌的组成以及各类病原菌对抗菌药物的耐药性,为临床医生合理选用抗菌药物解决患者病痛提供依据。方法收集临床送检的血培养标本,应用法国生物梅里埃公司BacT/Alert3D全自动血培养仪、配套培养基、鉴定仪系统鉴定菌种及药敏试验。结果 1764份血培养分离出病原菌179株,其阳性率为10.1%,其中革兰阳性菌68株(38.0%),革兰阴性菌111株(62.0%);病原菌中占首位的是大肠埃希菌,其次是凝固酶阴性菌、肺炎克雷伯菌、铜绿假单胞菌。药敏结果显示绝大部分检出的病原菌均有耐药情况出现。结论临床医生应重视患者早期的血培养检验,并严格根据药敏结果合理使用抗菌药物。对血培养的检测应更加规范,从而提高病原菌的检出率,并及时检测病原菌变化及耐药趋势。

Objective To understand the composition of the common pathogens in blood culture and a variety of pathogens to antimicrobial agents in the hospital.To offer the basis for the rational use of antimicrobial drugs to solve the patients＇ pain.Methods The collection involved1 764 blood samples.France bioMerieux BacT/Alert3 D fully automatic blood culture instrument,supporting medium,identification system were used to identify the pathogens and susceptibility test.Results A total of 1 764 blood samples showed the culture positive rate 10.1%,and there were 179 isolated positive bacteria,among which gram-positive bacteria were 68（38.0%）,gramnegative bacteria 111（62.0%）,at the first place of pathogens as E.coli followed by coagulasenegative bacteria,Klebsiella pneumonia,Pseudomonas aeruginosa.The susceptibility results showed that most of the detected pathogens were resistant to different anti-bacteria agents.Conclusion Clinicians should pay attention to early blood culture of patients,and strictly stick to the rational use of antimicrobial agents based on susceptibility results.